人参皂苷Rg3、IFN-α治疗血吸虫病肝纤维化的肝脏电镜观察

Electronic Microscope Observation of Schistosomiasis-induced Hepatic Fibrosis Treated by Ginsenoside Rg3 or IFN-α

目的观察人参皂苷Rg3、IFN-α抗血吸虫病肝纤维化的肝脏超微结构改变.方法将48只雄性IcR小鼠随机分为健康对照组(A组)、实验对照组(B组)、人参皂苷Rg3治疗组(C组)及IFN-α治疗组(D组)等4组,每组12只.以腹部贴片法感染B、C、D各组小鼠,每鼠感染血吸虫尾蚴18条～22条.肝纤维化造模10周,给予B、C、D各组小鼠吡喹酮杀虫治疗.以人参皂苷Rg3治疗C组小鼠、IFN-α治疗D组小鼠,用药8周后,常规方法制作肝脏组织切片HE染色和VG染色标本及投射电镜标本并观察,比较治疗后小鼠肝脏组织超微结构变化.结果 2治疗组(C、D组)小鼠肝窦周隙中胶原纤维较实验对照组明显减少,未见肌成纤维细胞,但可见贮脂细胞且数量比正常对照组增加;Rg3治疗组肝血窦扩张明显;IFN-α治疗组胞浆内可见滑面内质网增生.结论人参皂苷Rg3和IFN-α抗血吸虫病肝纤维化作用可能与促进肌成纤维细胞向贮脂细胞转变有关.

Objective To observe ultrastructure changes of schistosomiasis-induced hepatic fibrosis treated by ginsenoside Rg3 and IFN-α,respectively.Methods 48 ICR-strain male mice were divided into 4 groups named as normal control group（A）,infected control group（B）,Rg3 treated group（C） and IFN-α treated group（D）.There were 12 mice in each group.Mice in groups B,C and D were infected with 18 ~ 22 cercariae of S.japonicum.At 10 weeks post-infection,each mouse in groups B,C and D was treated by praziquantel（PZQ）.At the second day of PZQ treatment,mice in group C were treated by ginsenoside Rg3 and mice in group D were treated by IFN-α for 8 weeks.After 8-week treatment,liver tissue samples of these mice were taken and observed by microscopy and electron microscopy.Results The collagen depositions in disse spaces were significantly reduced in mice of both treated groups C and D compared with the untreated group B.Furthermore,myofibroblasts was not observed in the samples from the 2 treated groups,but noticed more fat-storing cells in them than those of group A sample.The hepatic portal areas were expanded in the samples from the Rg3 treated group and the smooth endoplasmic reticulum were hypertrophied in the samples from the IFN-α treated group.Conclusions The promotion of myofibroblasts transforming into fat-storing cells might related to the mechanism of anti-hepatofibrosis effects of Ginsenoside Rg3 and IFN-α on schistosomiasis-induced hepatic fibrosis in mice.