实时定量PCR的数据归一化方法

Normalization Method in Real-time PCR Data

在生命科学研究中,随着实时定量PCR(Real-time PCR)成为高通量快速、定量检测mRNA、micRNA等的常用技术,选择合适的数据归一化处理基准,对提高结果分析的准确性尤为重要.新近研究显示应根据不同研究目的或研究对象选用合适的Real-time PCR数据归一化方法,如单个内参基因归一化、多个内参基因归一化、micRNA归一化以及体外人工合成基因归一化等.就近年实时定量PCR的数据归一化方法进展做一综述.

In biological research,gene analysis with real-time PCR is a routine tool becoming increasingly important for high throughput and accurate profiling of mRNA,micRNA,etc.In real-time PCR,a right normalization count is particularly important for improving the accuracy of data analysis and results.In recent studies,appropriate normalizaiton reference in real-time PCR should be selected according to different objectives,such as single internal control gene normalization,multiple internal control genes normalization,microRNA normalizaiton and artificial molecular normalization and so on.In this paper,an overview of the progress of normalization in real-time PCR was presented.