大鼠肝缺血再灌注模型建立与评价

Rat model of hepatic ischemia-reperfusion injury

目的建立大鼠肝缺血再灌注模型,探讨该模型的制备及评价方法。方法大鼠麻醉、开腹后用血管夹钳夹支配肝左叶、中叶的肝蒂,造成70%肝脏缺血,1 h后放开血管夹恢复血供造成缺血再灌注损伤。根据再灌注时间不同分为2 h组、4 h组和24 h组,假手术组不进行钳夹肝蒂操作,观察各组肝脏生化指标及组织形态学变化。结果整个手术过程大鼠均能耐受。与假手术组比较,缺血再灌注2、4、24 h组大鼠血清中谷丙转氨酸(ALT)、谷草转氨酶(AST)、肝组织匀浆MDA水平以及肝脏病理组织学Suzuki's评分均明显升高,且在4 h组达到最高(P<0.05)。结论缺血1 h后再灌注4 h可制备最佳的肝缺血再灌注模型。该模型操作简单,为临床肝缺血再灌注损伤研究提供了基础。

Objective To investigate the hepatic ischemia-reperfusion injury in rats. Methods According to the reperfusion time, the rats were divided into four groups： sham, ischemia-reperfusion 2 h, 4 h, and 24 h groups. The blood vessels supplying the median and left lobe were occluded by micro-vascular clamp to achieve 70% liver ischemia for 1 h. The changes of liver biochemical markers and pathology were assessed. Results The survival rate was 100%. Compared with the sham group, the hepatic 70% ischemia-reperfusion resuhed in severe liver injury with marked elevation in serum aspartate aminotransferase, alanine aminotransferase, tissue malondialdehyde and histological scores （P〈0.05）. And after 4 h reperfusion, the insult was maximal. Conclusions It is recommended to make the optimal hepatic ischemia- reperfusion injury with 4 h reperfusion following ischemia for 1 h. Therefore, this model is useful for the research of hepatic ischemia-reperfusion injury, which is technically simple.