家兔泛素样小分子修饰因子-2基因克隆与序列分析

Cloning and sequence analysis of small ubiquitin-like modifier-2 (SUMO-2) gene from rabbit

目的克隆家兔泛素样小分子修饰因子-2(SUMO-2)基因,并预测其蛋白质结构和功能。方法应用分子克隆技术从家兔肝组织中克隆SUMO-2基因,用DNAMAN、CBS等生物软件及网络分析平台分析克隆所得到的SUMO-2基因序列及其编码的蛋白结构。结果测序结果显示成功克隆家兔SUMO-2基因片段,序列分析显示氨基酸序列与其他物种源性SUMO-2同源性较高,家兔SUMO-2蛋白氨基酸序列相对分子质量为10 826.9,等电点为4.70,蛋白主要以无规则卷曲形式存在(56.84%),可能含有1个N-糖基化位点,2个N-豆蔻酰化位点,1个丝氨酸磷酸化位点,2个苏氨酸磷酸化位点及2个蛋白激酶C磷酸化位点。无信号肽区域,无跨膜区,该蛋白可能为可溶性蛋白。结论成功克隆家兔SUMO-2基因,为进一步研究SUMO-2与肝纤维化的关系及其功能奠定基础。

Objective To clone the small ubiquitin-like modifier-2 （SUMO-2） gene from rabbit and predict its structure and function. Methods SUMO-2 gene was cloned from rabbit liver tissue and sequenced. Protein structure was predicted and analyzed by bioinformatics softwares, DNAMAN, CBS, and internet server. Results The whole gene sequence of SUMO-2 was obtained successfully by gene cloning from rabbit liver. The sequence showed a high homology with that of other species. The predicted molecular weight of SUMO-2 protein was 10 826.9, and the theoretical PI was 4.70. Random coil accounted for 56.84% in the secondary structure of SUMO-2. The protein was predicted to contain one N- glycosylation site, 2 N-myristoylation sites, one serine phosphorylation site, 2 threonine phosphorylation sites, and 2 protein kinase C phosphorylation sites. No signal peptide and transmembrane helices were found in the structure. This protein was predicted to be a soluble protein. Conclusion The successful cloning of SUMO-2 gene could provide the basis for the function study of SUM0-2 in hepatic fibrosis.