肝螺杆菌感染与人类原发性肝细胞癌的相关性研究

Identification of Helicobacter hepaticus in human bile samples of patients with primary hepatocellular carcinoma

目的本研究旨在了解肝肠源性螺杆菌是否存在于肝细胞癌患者的胆汁内,探讨此菌与肝癌的相关性。方法收集经病理确诊的47例原发性肝细胞癌患者及24例结肠直肠癌肝转移患者的胆汁标本。使用特定16SrRNA引物的聚合酶链式反应(PCR)及DNA测序检测胆汁标本中的螺杆菌属及肝螺杆菌特异性16SrRNA,并使用酶联免疫吸附法(ELISA)检测胆汁标本中的肝螺杆菌抗体。结果 47例原发性肝细胞癌患者胆汁标本中有12例(25.5%)检测出肝螺杆菌特异性16SrRNA,15例(31.9%)检测出抗肝螺杆菌抗体;24例结肠直肠癌肝转移患者胆汁标本中只有1例(4.2%)检测出肝螺杆菌特异性16SrRNA基因,3例(12.5%)检测出抗肝螺杆菌抗体(P<0.05)。结论原发性肝细胞癌患者可检测出肝螺杆菌,这可能与肝细胞癌的发病机制有一定联系。

Aim The aim of this study is to investigate whether H. hepaticus can be detected in the bile samples of patients with primary hepatocellular carcinoma （HCC）, and to investigate the relationship between H. hepaticus and HCC. Methods Bile samples were collected from 47 patients with HCC and 24 patients with hepatic metastasis from colorectal cancer diagnosed by histopathology. These samples were screened for the presence of enterohepatic Helicobacter spp. DNA was examined using polymerase chain reaction （PCR） with specific 16S rRNA primers, and sequencing. Bile samples were also examined for the presence of H. hepaticus antibody using by enzyme-linked immunosorbent assay （ELISA）. Results H. hepaticus DNA was found in 12 of 47 （25.5%） bile samples from patients with HCC, and in 1 of 24 （4.2%） samples from patients with liver metastatic tumor（P 〈 0.05）. Anti-H. hepaticus antibody was found in 15 of 47 （31.9%） bile samples from pa- tients with HCC, and in 3 of 24 （12.5%） samples from patients with liver metastasis （P 〈 0.05）. Conclusion H.hepaticus infection might play an important role in the development of primary hepatocellular carcinoma.