戊己丸不同配伍方对炎症后肠易激综合征模型大鼠结肠运动及5-羟色胺含量的影响

Influence of Wuji Wan in different compatibilities on colonic motility and 5-hydroxytryptamine contents in rats with post-infectious irritable bowel syndrome

目的:评价戊己丸不同配伍方对炎症后肠易激综合征(post-infectious irritable bowelsyndrome,PI-IBS)模型大鼠结肠运动功能的作用,并从5-羟色胺(5-hydroxytryptamine,5-HT)角度初步探讨其作用机制.方法:采用乙酸灌肠加束缚应激建立PI-IBS大鼠模型,应用戊己丸不同配伍方进行干预,应用BIOPAC MP150型多导生理记录仪描记大鼠结肠运动曲线,计算结肠运动指数和运动指数变化率,同时利用高效液相法检测血清、结肠、海马、下丘脑和额叶中5-HT的含量及5-HT转化率,甲苯胺蓝染色法计算结肠肥大细胞数目和脱颗粒率.结果:经戊己丸治疗后,中、高剂量组PI-IBS模型大鼠结肠运动指数(1770.10、1504.97、1700.64、1467.22 vs 2112.15)和运动指数变化率(68.10、40.16、59.97、39.33 vs 104.69)均显著下降(P<0.01).结肠组织中5-HT含量显著下降(3493.38、2640.41、2086.08、3255.63、2688.69、2129.13 vs 4168.36),血清中5-HT转化率(3.20、4.60、6.61、2.86、3.40、4.05 vs2.08)明显升高(P<0.05,P<0.01).中、高剂量组中枢边缘系统中5-HT含量(243.16、295.03、250.28、303.61 vs 124.42;303.51、397.30、339.94、353.02 vs 198.58;260.87、302.75、254.65、298.92 vs 166.71)显著升高(P<0.01),5-HT转化率(134.69、98.61、130.57、95.87vs 281.91;209.43、184.55、189.56、186.75vs 262.01;109.36、86.52、115.41、88.47 vs268.36)明显下降(P<0.05,P<0.01).结肠黏膜肥大细胞数目(6.40、5.11、6.48、5.57 vs 10.47)和脱颗粒率(23.81、17.94、23.25、19.19 vs34.10)亦明显下降(P<0.01).结论:戊己丸不同配伍方改善PI-IBS模型大鼠结肠运动功能的作用机制可能是通过改善5-HT含量及肥大细胞的异常状态,从而调节脑-肠轴功能实现的.

AIM：To evaluate the effect of Wuji Wan in different compatibilities on colonic motility and 5-hydroxytryptamine（5-HT） contents in rats with post-infectious irritable bowel syndrome （PI-IBS）.METHODS：A rat model of PI-IBS was established by intracolonic instillation of acetic acid and restraint stress.Rats were divided into several groups.Wuji Wan in different compatibilities was given to rats in Wuji Wan groups,Pinaverium bromide tablets to rats in the positive group and water to the other two groups.BIOPAC MP150 polygraph was used to record colon motion curves and evaluate colonic motility using motility index and the change rate of the motility index.HPLC-ECD was used to determine the contents of 5-HT and the percent conversion of 5-HT in serum,colon,odobenus rosmarus,hypothalamus and frontal lobe.The number of mast cells was counted to calculate their degranulation rate by Toluene ammonia blue staining.RESULTS：After treatment,the colonic motility index（1770.10,1504.97,1700.64,1467.22 vs 2112.15） and the change rate of motility index（68.10,40.16,59.97,39.33 vs 104.69） in middleand high-dose Wuji Wan groups were significantly decreased（all P0.01）.The content of 5-hydroxytryptamine in the colon（3493.38,2640.41,2086.08,3255.63,2688.69,2129.13 vs 4168.36） was remarkably reduced（all P0.01） and the conversion rate of 5-HT in serum（3.20,4.60,6.61,2.86,3.40,4.05 vs 2.08） was notably increased（all P0.05 or 0.01）.In the middleand high-dose groups,the contents of 5-HT in the limbic system（243.16,295.03,250.28,303.61 vs 124.42;303.51,397.30,339.94,353.02 vs 198.58;260.87,302.75,254.65,298.92 vs 166.71） were increased（all P0.01） and the percent conversion of 5-HT（134.69,98.61,130.57,95.87 vs 281.91;209.43,184.55,189.56,186.75 vs 262.01;109.36,86.52,115.41,88.47 vs 268.36） was decreased（all P0.05 or 0.01）.The number of mast cells（6.40,5.11,6.48,5.57 vs 10.47） and their degranulation ratio（23.81,17.94,23.25,19.19 vs 34.10） were significantly decreased（all P0.01）.CONCLUSION：Wuji Wan in different compat-ibilities exerts therapeutic effects on colonic motility in rats with post-infectious irritable bowel syndrome possibly by improving the contents of 5-HT and the abnormal condition of mast cells and therefore modulating the function of the brain-gut axis.