小凹蛋白-1在乳腺癌组织中的表达及其与乳腺癌浸润转移的关系

Expression of caveolin-1 in breast carcinoma and its correlation with infiltration and metastasis of breast carcinoma

目的观察小凹蛋白-1(Caveolin-1)在人乳腺癌组织中的表达,探讨Caveolin-1的表达与临床病理因素和预后的相关性。方法运用免疫组织化学方法(Envision染色)和Western印迹法检测168例人乳腺癌及其配对癌旁正常乳腺组织病理标本中Caveolin-1的表达水平,采用Prism 5.0统计软件分析肿瘤组织Caveolin-1与临床病理因素及患者总生存时间的关系。结果 Caveolin-1在人乳腺癌组织中的表达明显低于乳腺纤维素瘤和癌旁正常组织(19.8%vs 53.8%vs95.0%,P<0.01);随临床分期的递增(P=0.044)、淋巴结转移的出现(P=0.039)以及HER-2表达(P=0.014)的增加,Caveolin-1表达水平逐渐降低,各组间有显著相关性;Caveolin-1表达阳性的乳腺癌患者,术后5年生存率较Caveolin-1表达阴性者高(P=0.018);Caveolin-1的表达与肿瘤直径、患者年龄、组织学分级及ER/PR的表达无显著相关性。结论Caveolin-1的表达与临床病理因素及预后有一定关系,其在乳腺癌发生、发展中可能起到肿瘤抑制因子的作用。

Objective To investigate the correlation between caveolin-1 expression and clinicopathological factors and prognosis in patients with human breast carcinoma. Methods Using immunnohistochemistry to analyze the caveolin-1 expression in 168 patients with human breast carcinoma and adjacent normal breast tissue. The relationship of caveolin-1 expression and clinicopathological factors were analyzed using statistical software Prism 5.0. Results The expression of caveolin-1 in breast carcinoma was significantly lower than that in adjacent normal tissues （P〈0.01）.The caveolin-1 expression was significantly correlated with advancing clinical stage（P=0.044）,presence of maxillary lymph node matastasis（P=0.039）,and the increase of HER-2 positive（P=0.014）. The difference between groups was statistically significant （P〈0.01）.The patients with breast carcinoma and positive expression of caveolin-1 had a higher five-year survival rate（P=0.018）. The expression of caveolin-1 was not correlated with tumor size,age of the patients and histological grade. Conclusions The expression of caveolin-1 may be involved in the occurrence and development process of breast carcinoma.The expression of caveolin-1 has a certain relationship with clinicopathological factors in breast carcinoma. It may play a role of tumor inhibiting factor in breast carcinoma progression.