HPLC测定柴胡口服液中柴胡皂苷a、d含量方法的改进

Determination of Saikosaponin a,d in Radix Bupleuri Oral Liquids by Improved HPLC

目的:改进HPLC测定柴胡口服液中柴胡皂苷a、d含量的方法。方法:采用Purospher STAR C_(18)色谱柱(4.6 mm×250 mm,5μm);流动相:甲醇-0.05%磷酸(80:20);流速:1.0 ml·min^(-1);柱温:35℃;检测波长为210 nm。结果:柴胡皂苷a线性范围为0.041 92～2.096μg,r=0.999 9,平均回收率99.08%,RSD为0.40%;柴胡皂苷d线性范围为0.032 18～1.609μg,r=0.999 8,平均回收率98.27%,RSD为0.55%。结论:该法灵敏、简便、准确,适用于市售柴胡口服液中柴胡皂苷a、d的含量测定。

Objective：To improve the HPLC method for the determination of saikosaponin a,d in saikosaponin oral liquids.Method： A Purospher STAR C_（18） column（4.6×250 mm,5μm） was used,the mobile phase was methanol-0.05%phosphoric acid（80： 20）,the flow rate was 1.0 mL·min^（-1） 1,the column temperature was 35℃and the detection wavelength was 210nm.Result：The linear range of saikosaponin a was 0.041 92-2.096 p,g（r =0.999 9） with the average recovery of 99.08%（RSD =0.40%）.The linear range of saikosaponin d was 0.032 18-1.609μg（r= 0.999 8） with the average recovery of 98.27%（RSD =0.55%）.Conclusion： The established method is sensitive,simple,accurate and applicable in the content determination of saikosaponin a,d in the marketed Radix Bupleuri oral liquids.