摘要
目的:探讨基质金属蛋白酶-8(MMP-8)及其抑制因子-1(TIMP-1)在侵袭性牙周炎(AgP)牙龈组织中的表达情况。方法:采用免疫组织化学方法检测15例AgP患者和20例对照的牙龈组织中MMP-8和TIMP-1蛋白的表达情况。结果:MMP-8和TIMP-1在2组牙龈组织的上皮内及上皮下结缔组织中均有表达。AgP组MMP-8表达水平和MMP-8/TIMP-1均高于对照组(Z=2.990和4.911,P<0.05),2组TIMP-1的表达差异无统计学意义(Z=1.822,P>0.05)。ROC曲线分析结果显示,AUC(MMP-8)=0.752(95%CI=0.618～0.885,P=0.003),AUC(MMP-8/TIMP-1)=0.913(95%CI=0.831～0.996,P<0.001),AUC(TIMP-1)=0.347(95%CI=0.194～0.499,P=0.068)。结论:牙龈组织中MMP-8表达水平和MMP-8/TIMP-1可以作为诊断AgP的潜在指标。
Aim ： To study the expression of matrix metalloproteinase-8 （MMP-8） and its inhibitory factor-1 （ TIMP-1 ） in gingival tissue of patients with aggressive periodontitis（AgP）. Methods： Immunohistochemistry method was used to de- tect MMP-8 and TIMP-1 protein in gingival tissue of 15 AgP patients and 20 healthy control. Results： MMP-8 and TIMP-1 were positively expressed in the intraepithelial and subcutaneous connective tissue in both groups. MMP-8 expression level and MMP-8/TIMP-1 of AgP group were significantly higher than those of the control group （Z =2.990 and 4. 911 ,P 〈 O. 05 ）, but the difference in TIMP-1 expression between the 2 groups was not significant （ Z = 1. 822, P 〉 0.05 ）. ROC curve analysis showed that AUC（ MMP-8 ） = 0. 752 （ 95% CI = 0. 618 - 0. 885, P = 0. 003 ）, AUC （ MMP-8/TIMP-1 ） = 0.913（95% CI=O. 831 -0. 996,P 〈0. 001 ） ,AUC（TIMP-1 ） = 0. 347（95% CI =0. 194 - 0. 499,P = 0.068）. Conclu- sion： MMP-8 expression level and MMP-8/TIMP-1 in gingival tissue could be helpful for the diagnosis of AgP.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2013年第3期366-369,共4页
Journal of Zhengzhou University(Medical Sciences)
基金
广东省科技计划项目00204390134483040
