大鼠肝窦内皮细胞扫描电镜制样方法的改良

Modified sample preparation method for scanning electron microscopy of rat liver sinusoidal endothelial cells

目的:改良大鼠肝窦内皮细胞扫描电镜制样方法,以期得到优质图像。方法:采用红细胞裂解液去除红细胞,然后样本划痕制作断裂面的方法制备电镜样本。结果:改良法可清晰观察肝窦内皮细胞的窗孔。结论:通过裂解红细胞和样本划痕的方法制备样本,可以获得优质肝窦内皮细胞扫描电镜图像。

Objective; To improve the sample preparation method for scanning electron microscopy of rat liver sinusoidal endothelial cells. Methods： The erythrocytes were removed by erythrocyte lysis buff- er. Samples were prepared by scratching method. Results. Fenestrae in rat liver sinusoidal endothelial cells could be observed clearly by the modified method. Conclusion- We can get scanning electron microscope image of rat liver sinusoidal endothelial cells with high quality by lysis of erythrocytes combined with sampie scratching.