WT1在晚期浆液性卵巢上皮癌中的表达及意义

The expression and prognostic value of WT1 in advanced serous epithelial ovarian carcinoma

目的:确定晚期浆液性卵巢上皮癌存活与WT1基因表达的相关性。方法:收集2002～2010年经过完整术后化疗的晚期浆液性卵巢上皮癌女性患者168例,最终纳入研究109例。收集入选病例的完整病历资料及石蜡包埋的组织块切片后,WT1免疫染色并据此分组,采用卡普兰-迈耶曲线及COX回归法进行生存分析。结果:55例患者的病理组织WT1染色呈阳性,54例阴性。WT1阳性组的卵巢癌病灶大小和淋巴转移率显著高于WT1阴性组(P<0.05)。WT1阴性患者5年生存率为39.4%,WTI阳性患者5年生存率为10.7%,二者差异有统计学意义(P<0.05)。结论:WT1基因表达与晚期浆液性卵巢上皮癌预后不良密切相关。

Objective： To determine the survival and recurrence - free survival of women with advanced serous epithelial ovarian carcinoma in relation to WT1 gene expression. Methods： 168 patients who were diagnosed with advanced serous epithelial ovarian carcinoma and had undergone complete post - operative chemotherapy were selected, but 109 patients were selected into the final study group. The records of these women were reviewed and the paraffin - embedded tissues of these women were stained with WT1 immunostaining. Survival analysis was performed by using Kaplan - Meier and Cox regression methods. Results： Fifty - five patients showed WT1 positive staining and fifty - four did not. Size of residual tumor and lymphatic metastasis rate of WT1 positive expression group were higher than those of WT1 negative expression group （P 〈0. 05） . Five-year survival rate of positive and negative expression of WT1 were 39. 4% and 10. 7% , there was significant difference （P 〈 O. 05 ） . Conclusion： Expression of WT1 gene may be related to unfavorable prognosis of patients with advanced serous epithelial ovarian carcinoma.