期刊文献+

条件性恐惧消退大鼠内侧前额叶皮层边缘下区αCaMKⅡ的变化 被引量:1

The expression of α calmodulin kinase Ⅱ in infra-limbic cortex of medial prefrontal cortex of rats after fear extinction
下载PDF
导出
摘要 目的:探讨条件性恐惧消退早期(1周内)大鼠记忆保持成绩和内侧前额叶皮层边缘下区α钙调素依赖性蛋白激酶Ⅱ(αCaMKⅡ)的动态变化。方法:将70只成年雄性SD大鼠随机分为正常对照组、恐惧消退组和恐惧对照组,其中恐惧对照组为条件恐惧建立后不进行消退训练,恐惧消退组为条件恐惧建立24 h后进行消退训练,采用以声音为提示的足底电击建立大鼠恐惧记忆,然后仅给予声音信号进行恐惧消退训练。在恐惧消退后第1、3、7天分别进行消退保持测试,并在各时间点取边缘下区标本进行αCaMKⅡ的免疫组织化学检测。结果:在消退保持过程中,恐惧对照组和恐惧消退组大鼠消退成绩整体呈上升趋势,但均低于正常对照组,恐惧消退组消退成绩好于恐惧对照组(P<0.01);恐惧对照组和恐惧消退组大鼠边缘下区αCaMKⅡ免疫反应阳性物质逐渐增加,但均低于正常对照组,第1天无明显不同,第3、7天恐惧消退组αCaMKⅡ免疫反应阳性物质均低于恐惧对照组(P<0.01)。结论:恐惧消退训练后3~7 d,边缘下区αCaMKⅡ水平变化与恐惧记忆消退训练相关。 Objective: To observe the expression change of α calmodulin kinase Ⅱ(αCaMK Ⅱ )in infra-limbic cortex of medial prefrontal cortex of adult rats in the early stage of fear extinction. Methods:Seventy rats were randomly divided into normal control ,fear extinction and fear control group. The fear extinction training was not implemented in fear control group. The fear extinction group were trained to extinct fear with sound signal at 24 hours after fear established by sound hint of planter electric shock. The expressions of αCaMKⅡ in infra-limbic cortex were detected by immunohistochemistry at 1,3 and 7 days after fear extinction. Results: The expression of αCaMK Ⅱ in fear extinction and fear control group increased gradually, which was lower than that in normal control group. The fear extinction group were better than that in fear control group (P 〈 0.01 ). The immunoreactivities of αCaMKⅡ in infra- limbic cortex of fear extinction and fear control group increased gradually, which were lower than that in normal control group. The immunoreactivities of αCaMK Ⅱ in fear extinction group were lower than that in fear control group at 3 and 7 days after fear extinction (P 〈 0.01 ),but no significant difference at day 1. Conclusions: The αCaMK Ⅱ level in infra-limbic cortex is consistent with the extinction memory at 3 and 7 days after fear extinction training.
出处 《蚌埠医学院学报》 CAS 2013年第4期381-383,共3页 Journal of Bengbu Medical College
基金 安徽省高校省级科学研究基金资助项目(KJ2011B099)
关键词 应激障碍 创伤后 恐惧消退 α钙调素依赖性蛋白激酶Ⅱ 前额叶皮层 边缘下区 stress disorder, post-traumatic fear extinction α calmodulin kinase Ⅱ medial prefrontal cortex infra-limbic cortex
  • 相关文献

参考文献7

  • 1Heim C, Nemeroff CB. Neurobiology of posttraumatic stress disorder [ J ]. CNS Spectr,2009,14 ( 1 Suppl 1 ) : 13 - 24.
  • 2Kim SC, Jo YS, Kim IH, et al. Lack of medial prefrontal c.ortex activation underlies the immediate extinction deficit [ J ]. J Neurosci ,2010,30 ( 3 ) : 832 - 837.
  • 3Wan H, Mackay B, Iqbal H,et al. Delayed intrinsic activation of an NMDA-independent CaM-kinase Ⅱ in a eritieal time window is necessary for late consolidation of an associative memory [ J ]. J Neurosci ,2010,30( 1 ) :56 -63.
  • 4Cao X, Wang H. Inducible and selective erasure of memories in the mouse brain via chemical-genetic manipulation [ J ]. Neuron ,2008, 60(2) :353 -366.
  • 5Bayer KU, De Koninck P, Leonard AS, et al. Interaction with the NMDA receptor locks CaMK Ⅱ in an active conformation [ J ]. Nature,2001,411 (6839) :801 - 805.
  • 6韦美,李敏,李培培,张丽丽,汪涛.条件性恐惧消退早期大鼠边缘下区NR2B的动态变化[J].第三军医大学学报,2010,32(3):220-223. 被引量:3
  • 7Ledgerwood L, Richardson R, Cranney J. Effects of D-cycloserine on extinction of conditioned freezing [ J ]. Behav Neurosci, 2003, 117(2) :341 -349.

二级参考文献14

  • 1Milad M R,Rauch S L, Pitman R K, et al. Fear extinction in rats : implications for human brain imaging and anxiety disorders[J]. Biol Psychol, 2006,73(1) :61 -71.
  • 2Santini E, Ge H, Ren K, et al. Consolidation of fear extinction requires protein synthesis in the medial prefrontal cortex[J]. J Neurosci, 2004, 24(25) : 5704 -5710.
  • 3Milad M R, Wright C I, Orr S P, et al. Recall of fear extinction in humans activates the ventromedial prefrontal cortex and hippocampus in concert[J]. Biol Psychiatry, 2007, 62(.5) : 446 -454.
  • 4Hugues S, Garcia R. Reorganization of learning-associated prefrontal synaptic plasticity between the recall of recent and remote fear extinction memory[J]. Learn Mem,2007,14(8) :520 -524.
  • 5Quirk G J, Beer J S. Prefrontal involvement in the regulation of emotion: convergence of rat and human studies[ J]. Curr Opin Neurobiol, 2006, 16(6) : 723 -727.
  • 6Burgos-Robles A,Vidal-Gonzalez I, Santini E, et al. Consolidation of fear extinction requires NMDA receptor-dependent bursting in the yentromedial prefrontal cortex[ J ]. Neuron, 2007, 53 (6) : 871 - 880.
  • 7Mao Y, Zang S, Zhang J, et al. Early chronic blockade of NR2B subunits and transient activation of NMDA receptors modulate LTP in mouse auditory cortex[ J]. Brain Res, 2006, 1073 - 1074 : 131 - 138.
  • 8Rothbaum B O, Davis M. Applying learning plinciples to the treatment of post-trauma reactions [ J]. Ann NY Acad Sci, 2003,1008 : 112 - 121.
  • 9Maren S, Chang C H. Recent fear is resistant to extinction[J]. Proc Natl Acad Sci U S A, 2006, 103(47) :18020 -18025.
  • 10Stevenson C W, Meredith J P, Spicer C H, et al. Early life programming of innate fear and fear learning in adult female rats[J]. Behav Brain Res, 2009, 198( 1 ) :51 -57.

共引文献2

同被引文献21

  • 1侯彩兰,李凌江.前额叶-杏仁体神经环路在恐惧消退中的作用[J].中国行为医学科学,2006,15(5):472-474. 被引量:3
  • 2Davis M,Ressler K,Rothbaum B O,et al.Effects of D-Cycloserine on extinction:Translation from preclinical to clinical work[J].Biol Psychiatry,2006,60:369-375.
  • 3Gunduz-Cinar O,MacPherson K P,Cinar R,et al.Convergent translational evidence of a role for anandamide in amygdala-mediated fear extinction,threat processing and stress-reactivity[J].Mol Psychiatry,2013,18(7):813-823.
  • 4Makkar S R,Zhang S Q,Cranney J.Behavioral and neural analysis of GABA in the acquisition,consolidation,reconsolidation,and extinction of fear memory[J].Neuropsychopharmacology,2010,35:1625-1652.
  • 5Quirk G J,Russo G K,Barron J L,et al.The role of ventromedial prefrontal cortex in the recovery of extinguished fear[J].J Neurosci,2000,20(16):6225-6231.
  • 6Herry C,Garcia R.Prefrontal cortex long-term potentiation,but not long-term depression,is associated with the maintenance of extinction of learned fear in mice[J].J Neurosci,2002,22(2):577-583.
  • 7Milad M R,Quirk G J.Neurons in medial prefrontal cortex signal memory for fear extinction[J].Nature,2002,420(6911):70-74.
  • 8Holmes A,Singewald N.Individual differences in recovery from traumatic fear[J].Trends in Neurosciences,2012,36(1):23-31.
  • 9Barrett D,Shumake J,Jones D,et al.Metabolic mapping of mouse brain activity after extinction of a conditioned emotional response[J].J Neurosci,2003,23(13):5740-5749.
  • 10Pan Y W,Chan G C K,Kuo C T,et al.Inhibition of adult neurogenesis by inducible and targeted deletion of ERK5 mitogen-activated protein kinase specifically in adult nurogenic regions impairs contextual fear extinction and remote fear memory[J].J Neurosci,2012,32(19):6444-6455.

引证文献1

二级引证文献2

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部