虾青素高产突变株的选育

Studies on Astaxanthin-overproducing Mutants of Phaffia rhodozyma

阐述了法夫酵母中从葡萄糖到虾青素的整条生物合成途径。用亚硝基胍 (NTG)作为诱变剂 ,2 脱氧 D 葡萄糖 (2 DG)作为“筛子” ,通过 3轮诱变和一次自然分离 ,得到高产虾青素的菌株 4 2 6 ,其类胡萝卜素总量提高了 371 % ,为 7 0 7μg/mL ,虾青素的比率从 75 %提高到 79% ,并且其稳定性良好。对 2 DG和二苯胺作为筛子的作用机理也作了初步探讨。

The whole pathway of the biosynthesis from glucose to astaxanthin by P. rhodozyma is presented in this paper. The astaxanthin overproducing mutant 4 26 was obtained by means of NTG and 2 DG treatment. Compared with the parental strain L 0, the yield of total carotenoids was improved from 1.5?μg/mL to 7.07?μg/mL by 371%, the percentage of astaxanthin was improved from 75% to 79%. The genetic stability was good after ten generation. As a screen, the mechanism of 2 DG and dephenylamine were also analyzed preliminarily.