化痰中药半夏及山慈菇抗动脉粥样硬化的作用机制研究

Anti-atherosclerosis Effect and Mechanism of Phlegm-removing Herbs of Rhizoma Pinelliae and Pseudobulbus Cremastrae seu Pleiones

目的探讨半夏、山慈菇的调脂及内皮保护作用。方法采用高脂喂养方法复制大鼠高脂血症模型,造模成功后,将高脂血症大鼠随机分为模型组、阿托伐他汀组、半夏组及山慈菇组4组,分别给予生理盐水、阿托伐他汀、半夏水提液及山慈菇水提液,每天1次,连续灌胃给药12周,并继续给予高脂喂养;将非高脂喂养大鼠设为正常对照组。检测比较各组大鼠的血脂及血清内皮素-1(ET-1)、细胞间黏附因子(ICAM-1)、单核细胞趋化蛋白(MCP-1)水平。结果阿托伐他汀组、半夏组、山慈姑组血清总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)水平均显著下降,差异有统计学意义(P<0.05),半夏组、山慈菇组及阿托伐他汀组的ET-1、MCP-1及ICAM-1水平虽较模型组降低,但仅有半夏组及阿托伐他汀组的ET-1水平的降低差异有统计学意义(P<0.05)。结论半夏与山慈菇均有降低血脂的作用,半夏还可通过调节ET-1起到保护内皮的作用。

Objective To explore the effect of phlegm-removing herbs of Rhizoma Pinelliae and Pseudobulbus Cre mastrae seu Pleiones on regulating blood lipids and on protecting endothelium. Methods Hyperlipemia rat model was induced by high fat diet. After modeling, hyperlipidemia rats were randomly divided into four groups, model group, atorvastatin group, Rhizoma Pinelliae group and Pseudobulbus Cremastrae seu Pleiones group. The four groups were given saline, atorvastatin, Rhizoma Pinelliae water extract, Pseudobulbus Cremastrae seu Pleiones water extract, respectively, twice daily, continuous intragastric administration for 12 weeks. High fat diet was still kept on during the administration. Rats feeding with routine diet served as the blank control group. The levels of blood lipids, serum endothelin -1 （ET-1）, intercellular adhesion molecule （ICAM-1） and monocyte chemoattractant protein （MCP-1） were detected and compared. Results The levels of total cholesterol （TC） and low-density lipoprotein cholesterol （LDL-C） were significantly decreased in atorvastatin group, Rhizoma Pinelliae group and Pseudobulbus Cremastrae seu Pleiones group, the differences being significant as compared with the model group（P 〈 0.05）. Compare the mod- el group, the decrease of ET-1 in Rhizoma Pinelliae group and atorvastatin group had significantly defferences（P 〈 0.05 ）. Conclusion Phlegm-removing herbs of Rhizoma Pinelliae and Pseudobulbus Cremastrae seu Pleiones show certain effect on regulating blood lipids, and Rhizoma Pinelliae can protect endothelial through regulating ET-1 level.