清金得生片联合化疗对Lewis荷瘤小鼠Fas、FasL mRNA表达的影响

Effect of Qingjin Desheng Tablets Combined with Chemotherapy on Fas/FasL Gene Expression in Lewis Lung Cancer Mice

目的观察清金得生片联合化疗对Lewis荷瘤小鼠Fas、FasL mRNA表达的影响。方法 Lewis肺癌荷瘤小鼠随机分为模型组、清金组、顺铂组、清金加顺铂组,给药10 d后,取肿瘤组织,采用实时荧光定量PCR法检测各组小鼠Fas、FasL mRNA的表达。结果清金加顺铂组瘤体组织Fas mRNA的表达显著高于模型组(P<0.05),各给药组间差异无统计学意义;与模型组比较,清金组、顺铂组、清金加顺铂组瘤体组织FasLmRNA的表达差异均有统计学意义(P<0.05),各给药组间差异无统计学意义。结论清金得生片联合化疗的抗肿瘤作用与调节Fas/FasL系统,从而逆转肿瘤的免疫逃逸有关。

Objective To observe the effect of Qingjin Desheng tablets （QDT） combined with chemotherapy on Fas/FasL gene expression in Lewis lung cancer mice. Methods Lewis lung carcinoma cells were transplanted into 40 C57BL/6J mice, and the tumor-bearing mice were randomly divided into model group, QDT group, Cisplatin group, and the combination group. After treatment for 10 days, the expression of Fas and FasL mRNA in the tumor tissue was dectected with real-time fluorescent quantitation PCR. Results The combination group had higher Fas mRNA expression level than the model group（P 〈 0.05）, but QDT group and Cisplatin group did not differ from the model group. The difference of FasL mRNA expression between the three medication groups and the model group was significant （P 〈 0.05）, while the difference between the three medication groups was insignificant. Conclusion QDT combined with chemotherapy can suppress Lewis lung cancer by regulating the balance of Fas/FasL, thus to resist tumor immune escape.