吴茱萸碱和吴茱萸次碱血浆蛋白结合率的测定

Determination of Plasma Protein Binding Rate of Evodiamine and Rutaecarpine

目的建立兔血浆中吴茱萸碱和吴茱萸次碱浓度的测定方法,测定吴茱萸碱和吴茱萸次碱的血浆蛋白结合率。方法采用平衡透析法测定吴茱萸碱和吴茱萸次碱血浆蛋白结合率,利用HPLC测定血浆中吴茱萸碱和吴茱萸次碱浓度。结果吴茱萸碱在0.208,1.04,2.08μg.mL-1浓度下的血浆蛋白结合率分别为(38.5±1.7)%、(36.4±1.0)%、(39.0±1.0)%,吴茱萸次碱在0.235,1.18,2.345μg.mL-1浓度下的血浆蛋白结合率分别为(40.6±3.2)%、(39.3±4.5)%、(41.5±1.2)%,吴茱萸碱和吴茱萸次碱的浓度对血浆蛋白结合率无显著影响。结论吴茱萸碱和吴茱萸次碱与兔血浆蛋白具有较低等强度的结合。

Objective To establish an analytical method for evodiamine and rutaecarpine concentration in rabbit plasma, and to determine the plasma protein binding rate of evodiamine and rutaecarpine. Methods The equilibrium dialysis method was adopted to determine evodiamine and rutaecarpine concentration in plasma, and HPLC was used to calculate rabbit plasma protein binding rate of evodiamine and rutaecarpine. Results The protein binding rate of evodiamine was （38.5 ± 1.7）%, （36.4 ± 1.0）% , （39.0 ± 1.0）% when the plasma concentration of evodiamine was 0.208, 1.04, 2.08 μg·mL^-1, and the protein binding rate of rutaecarpine was（40.6 ± 3.2）%, （39.3 ± 4.5）%, （41.5 ± 1.2）% when the plasma rutaeearpine concentration was 0.235, 1.18, 2.345 μg·mL^-1, respectively. The binding rates of evodiamine and rutaeearpine were not correlated with the plasma concentration. Conclusion Evodiamine and rutaecarpine have a lower protein binding rates with rabbits plasma.