摘要
目的建立苦参药材中黄酮类成分的HPLC指纹图谱方法,为科学全面评价该药材质量提供依据。方法采用HPLC法对不同产地的苦参药材进行分析。色谱条件为Alltech Apollo C18色谱柱(4.6 mm×250 mm,5μm),流动相为甲醇-0.2%甲酸水溶液系统,梯度洗脱,流速1.0 mL/min,检测波长310 nm,柱温25℃。结果确定了13个色谱峰(或峰组)为各产地药材特征共有峰,并利用对照品对照和LC-MS法对其中的6个共有峰进行了成分指认。结论该方法简便可靠,重复性好,能够表征苦参药材的内在信息,可用于苦参药材中黄酮类成分的质量控制。
Objective To establish a fingerprint analysis method of flavonoids in herbal medicine of Radix Sophorae by HPLC. Methods The analysis was performed on Alhech Apollo C18 column （4.6mm×250mm,5μm） with a gradient mobile phase of methanol-0.2 % formic acid solution system at flow rate of 1.0mL/min. The detection wavelength was 310 nm. The column temperature was set at 25 ℃. Results The fingerprint of flavonoids in Radix Sophorae was established, and 13 common peaks were obtained. Among them, 6 peaks were identified by comparison with the references and by LC-MS. Conclusion The method is quick, simple and reproducible, and can be used as an effective means of quality evaluation of flavonoids in herbal medicine of Radix Sophorae.
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2013年第3期282-285,共4页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
广东省自然科学基金项目(S2012040006855)
广东高校优秀青年创新人才培养计划项目(LYM10095
2012LYM_0038)
广东省中医药管理局科研项目(20122091)
关键词
苦参
黄酮类成分
指纹图谱
高效液相色谱法
Radix Sophorae
Flavonoids
Fingerprint
High performance liquid chromatography
