HPLC法同时测定肿节风提取物中绿原酸、异嗪皮啶、迷迭香酸的含量

Simultaneous Determination of Chlorogenic Acid,Isofraxidin and Rosmarinic Acid in Extract of Sarcandra glabra(Thunb.) Nakai by HPLC

目的建立运用HPLC法同时测定肿节风提取物中绿原酸、异嗪皮啶和迷迭香酸含量的方法。方法采用Agilent ZORBAX Eclipse XDB—C18（4．6mm×250mm，5μm）色谱柱；流动相：乙腈（A）-0．1％甲酸（B），梯度洗脱0～30min，5％→20％A；30～40rain，20％→25％A；40～60rain，25％→29％A；60～65min，29％→32％A；流速：1mL·min^-1；检测波长：330nm；柱温：30℃；进样量：20μL。结果绿原酸、异嗪皮啶、迷迭香酸分别在0．0280～0．448μg（r=0．9998）、0．0164—0．262μg（r=0．9999）、0．113—1．81μg（r=0．9999）范围内呈良好的线性关系；平均回收率（n=6）分别为103．4％（RSD=2．77％）、98.3％（RSD=2．58％）、95.2％（RSD=2．49％）。结论该法简便快速，重复性好，准确可靠，可用于同时测定肿节风提取物中绿原酸、异嗪皮啶、迷迭香酸的含量．

Objective To establish a method for simultaneous determination of chlorogenic acid, isofraxidin and ros-marinic acid in the extract of Sarcanclra glabra（Thunb.） Nakai. Methods The three components were separated on a Agilent ZORBAX Eclipse XDB-CIs column（4.6 mm × 250 mm, 5 μm） with a flow-rate of 1 mL·min^-1 at 30 ℃. The mobile phase consisted of acetonitrile and 0.1% formic acid. The gradient condition was A： 5 %→20 % from 0 to 30 rain, A： 20 %→25 % from 30 to 40min, A： 25 %→29 % from 40 to 60 min, A： 29 %→32 % from 60 -65min. The detection wavelength was set at 330 nm and the injection volume was 20 μL. Results The linear ranges of chlorogenic acid, isofraxidin and rosmarinic acid were 0.0280-0.448 μg（r=0.9998）, 0.0164-0.262 μg （r=0.9999）, 0.113-1.81 μg（r=0.9999）, respectively. The mean recoveries （n=6） of chlorogenic acid, isofraxidin and rosmarinic acid were 103.4 %（RSD=2.77 %）, 98.3 %（RSD=2.58 %）, 95.2 %（RSD=2.49 %）, respectively. Conclusion The established method is simple, reproducible, and accurate, and can be used for the simultaneous determination of chlorogenic acid, isofraxidin and rosmarinic acid in the extract of Sarcandrae glabra.