Cu^(2+)胁迫下三种蔬菜四种抗氧化生理指标的变化

Study on the Cu^(2+) Stress on Physiological and Biochemical Index in Three Kinds of Vegetables

以油菜、生菜、茼蒿的幼苗为试材,研究了不同浓度CuCl2溶液(0、40、80、120、160mg/L)胁迫下3种蔬菜的生理生化指标(SOD活性、POD活性、MDA含量、维生素C含量)的变化。结果表明:随CuCl2胁迫浓度的升高,3种蔬菜超氧化物歧化酶(SOD)、过氧化物酶(POD)活性均呈先升后降的趋势,活性变化主要由种间和CuCl2胁迫引起;丙二醛(MDA)含量呈逐渐上升趋势,含量变化主要由浓度引起;维生素C含量呈先降再升趋势,含量变化与种间和胁迫浓度都有关。试验推测,茼蒿的抗铜胁迫机制可能以SOD、POD活性平衡上升为主,油菜的抗铜胁迫机制可能与维生素C含量上升关系密切。

Three kinds of vegetables （rape, lettuce, chrysanthemum）seedlings was chosen as test materials, different concentrations of CuCl2 solution （0 mg/L, 40 mg/L, 80 mg/L, 120 mg/L, 160 mg/I.） stress on physiological and biochemical indexes （SOD activity,POD activity,MDA content,VC content） changes were studied. The results showed that with the stress concentration increasing, three kinds of vegetables SOD,POD activity showed an increasing and then decreasing trend and the changes of activity caused by the different vegetables ; the content of MDA was gradually rising trend and the changes of Content caused mainly by the concentration of cadmium stress;the content of VC decreased at first and then ascending trend and the changes of content caused by the different vegetables and concentration of cadmium stress. It was speculated that chrysanthemum of resistance to copper stress mechanism may be SOD, POD activity rised evenly and rape resistance to copper stress mechanism may be related to the content of VC increased close.