TUBB3基因在侵袭性非霍奇金淋巴瘤中的表达及临床意义

Expression and clinical significance of TUBB3 gene in invasive non-Hodgkin＇ s lymphoma

目的探讨TUBB3基因在侵袭性非霍奇金淋巴瘤中的表达及与患者临床特征和顸后的关系，为个体化治疗提供理论依据。方法选择侵袭性非霍奇金淋巴瘤患者65例的组织蜡块，采用Taqman逆转录一聚合酶链反应方法检测TUBB3基因的表达量，分析其与患者临床特征和预后的关系。统计学分析应用SPSS17．0软件。结果TUBB3基因在侵袭性非霍奇金淋巴瘤中高表达率为15．4％（10／65），弥漫大B细胞淋巴瘤和T细胞淋巴瘤中高表达率分别为28．1％（9／32）和3．O％（1／33）（x2=7．859，P=0．005）。在弥漫大B细胞淋巴瘤中TUBB3基因高表达患者与I临床特征的性别有关（X2=4．802，P=0．028），女性为77．8％（7／9），男性为22．2％（2／9）；高表达患者与低表达患者的总生存率分别为55．6％（5／9）和91．3％（21／23）（x2=5．426，P=0．020）。结论TUBB3基因在弥漫大B细胞淋巴瘤中高表达较T细胞淋巴瘤更为明显。在弥漫大B细胞淋巴瘤TUBB3基因高表达患者中女性高于男性，低表达者的总生存率高于高表达者。而在T细胞淋巴瘤中与临床特征及预后关系不明显。

Objective To investigate the ralations hip between the expression of TUBB3 gene and clinical characteristics, prognosis of invasive non-Hodgkin＇ s lymphoma, in order to provide theoreti- cal basis for individual therapy. Methods Sixty-five tissues of invasive non-Hodgkin＇ s lymphoma were choosed, Taqman RT-PCR was used to detect the expression quantities of TUBB3 gene, and the relations with clinical characteristics and prognosis was analyzed. SPSS 17. 0 statistical was used to analyze. Re- suits High expression rate of TUBB3 gene in invasive non-Hodgkin＇ s lymphoma was 15. 4% （10/65）, in diffuse large B cell lymphoma（DLBCL） was 28. 1% （9/32） and in T-cell lymphoma was 3. 0% （1/33） （X2 = 7. 859, P = 0. 005 ）. High expression of TUBB3 gene was related with gender in DLBCL （X2 = 0. 802, P = 0. 028）, female rate was 77. 8% （7/9）, male rate was 22. 2% （2/9） ; Overal survive（OS） ates of high expression and low expression was 55. 6% （5/9） and 91.3% （21/23）（X2 =5. 426, P = 0. 020）. Conclusions Higher expression of TUBB3 gene is obvious in DLBCL than that in T-cell lympho- m. In the high expression of patients, fema/e is higher than male in DLBCL; OS of low expression is high- ： than that of high expression. Clinical characteristics and pronosis are not obvious in T-cell lymphoma.