高效液相色谱法同时测定丹皮总苷部位3种单萜苷类

Simultaneous determination of three monoterpene glycosides in total glucosides of Mudan cortex by HPLC

目的:建立同时测定丹皮总苷部位氧化芍药苷、芍药苷、苯甲酰芍药苷含量的方法。方法:采用Shim-pack VP-ODSC18柱,以乙腈-0.02%磷酸水为流动相,流速为1.0mL.min-1,检测波长为230nm。结果:氧化芍药苷、芍药苷、苯甲酰芍药苷的线性范围分别为0.015 7～0.157 0mg.mL-1(r=0.999 8)、0.044 6～0.446 0mg.mL-1(r=0.999 6)、0.007 4～0.073 6mg.mL-1(r=0.999 6);平均加样回收率分别为99.55%,99.37%,101.22%,RSD分别为0.90%,1.24%,1.18%。结论:该方法准确、可靠,重复性好,可用于丹皮总苷及其制剂的质量分析。

OBJECTIVE To establish a method for simultaneous determination of oxypaeoniflorin,paeoiflorin,benzoylpaeoniflorin in total glucosides of Mudan cortex.METHODS The determination was performed on a Shim-pack VP-ODS C18 column with acetonitrile-0.02% phosphoric acid as the mobile phase.The flow rate was 1.0 mL·min-1 and the detection wavelength was 230 nm.RESULTS The oxypaeoniflorin,paeoiflorin,benzoylpaeoniflorin showed a good relationship in the ranges of 0.015 7~0.157 0 mg·mL-1（r=0.999 8）,0.044 6~0.446 0 mg·mL-1（r=0.999 6）,0.007 4~0.073 6 mg·mL-1（r=0.999 6）.The average recoveries of oxypaeoniflorin,paeoiflorin,benzoylpaeoniflorin were 99.55%,99.37%,101.22% and RSD were 0.90%,1.24%,1.18%,respectively.CONCLUSION The method is reliable,accurate,reproducible and can be applied to the quality control of total glucosides of mudan cortex.