尿素包合法富集纯化杜仲籽油α-亚麻酸的工艺优化

Optimization of enrichment and purification technology of α-linolenic acid in Eucommia seed oil by the urea adduction fractionation method

采用二次正交旋转组合设计,对尿素包合法富集纯化杜仲籽油中α-亚麻酸的工艺进行了优化研究。在单因素实验的基础上,以α-亚麻酸纯度为考察指标,考察了尿素与脂肪酸质量比、95%乙醇与脂肪酸质量比、包合温度、包合时间等四个实验因素对α-亚麻酸富集效果的影响,建立了二次多元回归方程预测模型。实验结果表明α-亚麻酸最佳富集工艺条件为:尿素与脂肪酸质量比为3:1,95%乙醇与脂肪酸质量比为9:1,包合温度为-9.0℃,包合时间为17.0h。在此最佳富集条件下,α-亚麻酸纯度可提高至82.63%。尿素包合法是富集纯化杜仲籽油α-亚麻酸的有效方法。

Quadratic rotation- orthogonal combination design was applied to optimize the enrichment and purification techniques of α-linolenic acid in Eucommia ulmoides seed oil by the method of urea adduction fractionation.By taking α-linolenic acid purity as the investigation index,the effects of four process parameters named ratio of urea to fatty acid,ratio of 95% ethanol to fatty acid,inclusion temperature and inclusion time on the enrichment results were investigated and the two times multiple regression equation model of 5-linolenic acid purity was established based on the single factor experiments.The results showed that the optimum levels of the four parameters were as follows. 3： 1 ,9：1,-9.0℃ and 17.0h, respectively. Under the optimum enrichment conditions, the purity of 5-linolenic acid was up to 82.63%.Urea adduction fractionation was an effective method for the enrichment of a-linolenic acid in Eucommia ulmoides seed oil.