番茄红素对CCl_4诱导的正常肝细胞株HL-7702损伤的影响

Effect of lycopene on hepatocyte injuries induced by carbon tetrachloride

目的体外观察番茄红素对人正常肝细胞株HL-7702细胞活力及凋亡的影响,探讨其对肝细胞的保护作用。方法体外培养人正常肝细胞株HL-7702,建立CCl4肝损伤模型,终浓度为2、5、10μmol·L-1的番茄红素干预处理,MTT法测定细胞活性、Horchest33342染色及TUNEL检测观察细胞凋亡。结果番茄红素在2～10μmol·L-1浓度范围内的对肝细胞无毒性,可抑制CCl4所致肝细胞活性下降,减少CCl4所致的肝细胞凋亡,一定剂量范围内与番茄红素浓度相关。结论番茄红素对正常肝细胞HL-7702具有保护作用。

Objectives To observe the effect of lycopene on the growth and apoptosis of HL-7702 hepatocyte strain and to explore the protection on hepatocyte. Methods The exoteric hepatic injuries model, caused by carbon tetraehloride （ CCI4 ） , which was interfered by lycopene（ at concentrations of 2,5,10 μmol .L-1 ）, was established with well-cultivated HL-7702 hepatocyte strain. The growth conditions were detected by MTT method and apoptosis was observed with Horchest33342 and TUNEL staining. Results Toxicity to hepatocytes of lyeopene,at concentrations from 2 μmol .L-1 tol0 μmol . L-1 ,was not observed. Hepatocytes had some protective effects on the vigor impact induced, and could reduce the hepatic apoptosis provoked by CC14, and correlation with the concentrations of lycopene. Con- clusions Lycopene had certain protective effects on hepatocyte.