血清学IgM检测在儿童呼吸道感染中的临床意义

Clinical significant of serological IgM test on the respiratory tract infection among children

目的对呼吸道感染患儿的几种常见病原体进行血清学检测,从而评价血清学IgM检测的临床意义。方法选取360例呼吸道感染的患儿作为调查对象,用酶联免疫吸附测定(ELISA)法对血清IgM进行检测,测定患儿血清中CMV-IgM、HSV-IgM、EBV-IgM、RSV-IgM、MPM-IgM的情况,分析抗体阳性率、不同性别和不同年龄组抗体阳性率的差异。结果本次研究检测出IgM抗体阳性率为76.7%,其中,CMV-IgM阳性率为17.2%,HSV-IgM阳性率为9.7%,EBM-IgM阳性率为9.2%,RSV-IgM阳性率为20.8%,MPM-IgM阳性率为19.7%,各类抗体的阳性率比较差异有高度统计学意义(χ2=35.98,P<0.01)。男性患儿阳性率为72.4%,女性患儿阳性率为82.7%,男性患儿与女性患儿的阳性率差异无统计学意义(χ2=0.58,P>0.05)。<1岁组检测各类抗体合计IgM阳性率为85.3%,1-<4岁组为85.3%,4-6岁组为62.3%,>6岁组为78.0%,不同年龄组间抗体的阳性率差异具有高度统计学意义(χ2=19.14,P<0.01)。CMV-IgM、HSV-IgM及EBM-IgM的阳性率在不同年龄组间差异无统计学意义;RSV-IgM的阳性率在各年龄组间有差异(χ2=11.37,P<0.01),1-<4岁组的阳性率(37.04%)高于其他年龄组;MPM-IgM的阳性率在各年龄组间也有差异(χ2=28.48,P<0.01),>6岁组的阳性率(49.30%)高于其他年龄组。结论血清学IgM检测能够作为儿童呼吸道感染患儿的早期指标,在临床合理用药、降低患者不必要经济损失方面具有重要的临床意义。

Objective To conduct serological test on the respiratory tract infection among children,so as to evaluate the clinical effect of serological IgM test.Methods A total of 360 children were collected in our study.ELISA method was taken to perform the IgM test,and the CMV-IgM,HSV-IgM,EBV-IgM,RSV-IgM and MPM-IgM were also test ed.The positive rate of antibody of different genders and ages were analyzed.Results The positive rate of antibody IgM was 76.7%,of which the positive rate of antibody CMV-IgM was 17.2%,the positive rate of antibody HSV-IgM was 9.7%,the positive rate of antibody EBM-IgM was 9.2%,the positive rate of antibody RSV-IgM was 20.8%,and the positive rate of antibody MPM-IgM was 19.7%.There was significant difference among these positive rates（χ2= 35.98,P 0.01）.The positive rate of males was 72.4%,and was 82.7% among females.There was no significant dif ference between positive rates between males and females（χ2=0.58,P 0.05）.The positive rates of IgM of 0-1 years old age group,1-4 years old age group,4-6 years old age group and above 6 years old age group were 85.3%,85.3%,62.3% and 78.0% respectively.There was significant difference of positive rates among different age groups（χ2=19.14,P0.01）.There was no significant difference among positive rates of CMV-IgM,HSV-IgM and EBM-IgM.There was significant difference in the positive rates of different age groups（χ2=11.37,P 0.01）.The positive rate of 1-4 years old group（37.04%） was significant higher than other age groups.The positive rate of MPM-IgM showed significant difference in different age groups（χ2=28.48,P 0.01）.The positive rate of 6 years old group（49.30%） was significant higher than other age groups.Conclusion The serological IgM test could be an early indexes for respiratory tract infection among children,we could decrease the economic lose in clinics.