野鸦椿水提物对大鼠慢性肝纤维化的影响

目的研究野鸦椿水提物对慢性肝纤维化的作用及机制。方法将50只大鼠随机分为正常组、模型组、秋水仙碱组及野鸦椿高和低剂量组,除正常组外灌胃给予CCl4,2次/周,共8周,造成慢性肝纤维化模型。第4周开始秋水仙碱组给秋水仙碱0.1mg/kg,野鸦椿高、低剂量组分别给野鸦椿水提物1mL/kg和0.2mL/kg,连续5周,模型组及对照组给等体积生理盐水。给药结束后处死动物取血、分离血清,检测HA、LN、PⅢP及TGF-β1、TNF-α含量。结果野鸦椿高、低剂量组HA、LN、PⅢP及TGF-β1、TNF-α均明显低于模型组,高剂量组HA、LN浓度与秋水仙碱组无显著性差异,PⅢP则高于秋水仙碱组,而低于低剂量组;野鸦椿高剂量组TGF-β1浓度与秋水仙碱组无显著性差异,而低剂量组则高于秋水仙碱组,野鸦椿高剂量组则显著低于低剂量组;野鸦椿高、低剂量组TNF-α浓度均高于秋水仙碱组,野鸦椿高剂量组则显著低于低剂量组。结论野鸦椿水提物具有抗慢性肝纤维化的作用,并存在一定的量效关系,抗肝纤维化的作用可能与其抑制TGF-β1升高有关。

Objective To study the aqueous extract of Euscaphis effect and mechanism of chronic liver fibrosis. Methods 50 rats were randomly divided into normal group, model group, colchicine group and Euscaphis high-and low-dose group, in addition to the normal group, orally given CC14, 2 times / week for 8 weeks, resulting in chronic hepatic fibrosismodel. 4 th week, the colchicine group to colchicine 0.1 mg/kg, Euscaphis high and low dose groups respectively to extract 1 mL/kg and 0.2 mL/kg to Euscaphis water for five consecutive weeks, the model group and control The group was given an equal volume of saline. After administration, the animals were sacrificed blood, serum was separated, detection of HA, LN, P III P and TGF-β1, TNF-a content. Results The wild crow Tsubaki high, the low-dose group HA, LN, P III P and TGF-β1, TNF-a were significantly lower than the model group, no significant difference for the high-dose group HA, LN concentration with colchicine group, P III P is higher than autumn Narcissus alkali group, but lower than the low-dose group; Euscaphis high dose group TGF-β1 concentration of colchicine group was nosignificant difference between the low-dose group than colchicine group, Euscaphis high dose groupsignificantly lower than the low-dose group; Euscaphis, TNF-a concentration of the low-dose group were higher than the colchicine group, Euscaphis high dose group was significantly lower than the low-dose group. Conclusion Euscaphis aqueous extract of the role of anti-chronic liver fibrosis, and there is a dose-effect relationship, and anti- hepatic fibrosis may be related to inhibition ofTGF-β1 elevated relevant.