碲化镉量子点对CHL细胞染色体畸变作用研究

Effects of CdTe QDs on chromosome aberration of CHL cells in vitro

目的探讨碲化镉量子点(CdTe QDs)对中国仓鼠肺成纤维细胞(CHL)染色体畸变作用。方法用体外方法检测CdTe QDs在1.6、3.2、6.3、12.6、25.2μl/ml染毒浓度范围内,在代谢活化条件下(+S9)及非代谢活化条件下(-S9)CHL细胞的染色体畸变率。结果 CdTe QDs的代谢活化组(+S9)在染毒浓度为2.6μl/ml和25.2μl/ml浓度组CHL细胞染色体畸变率与对照组比较,显著增加(P<0.01),染色体畸变类型主要是染色体断裂、交换和碎片等。结论 CdTe QDs在代谢活化条件下(+S9)可能导致CHL细胞染色体畸变。

Objective To observed the chromosome oberration of CHL cells induced by CdTe QDs.Methods The chromosome oberration test of Chinese hamster lung（CHL） cell was conducted in CdTe QDs at the concentrations of 1.6,3.2,6.3,12.6 and 25.2μl/ml,under the metabolic（＋S9） and non-metabolic（-S9）activation systems.Results The chromosome oberration rate of 12.6 and 25.2μl/ml CdTe QDs groups were significantly higher than the control groups（P0.01） Under the condition of metabolic activation.Main types of chromosomal aberrations was broken,fragmented and exchange.Conclusion It was suggested that CdTe QDs could induce the effects of chromosome oberration under the metabolic activation systems（＋S9）.