拟康氏木霉菌丝多糖提取工艺优化

Optimization of Extraction of Polysaccharides from Trichoderma pseudokoningii Mycelium

目的:研究拟康氏木霉菌丝多糖的最佳提取工艺条件。方法:以多糖提取率为考察指标,通过单因素试验和正交试验研究拟康氏木霉多糖的最佳提取工艺。结果:影响拟康氏木霉多糖提取率的因素顺序为回流温度>提取次数>提取时间>粉碎粒度;最佳工艺条件为粉碎过120目筛,料液比1:30(g/mL),100℃沸水回流提取3次,每次2h,在此条件下,拟康氏木霉菌丝多糖得率为(11.09±0.13)%。结论:该工艺稳定可行,可作为拟康氏木霉菌丝多糖的提取条件。

Objective： To study the optimal extraction conditions of polysaccharides from Trichoderma pseudokoningii mycelium.Methods： One-factor-at-a-time experiments and orthogonal array design were applied to establish the optimum extraction conditions based on the yield of polysaccharide.Results： The influence of three factors on extraction efficiency decreased in the following order： reflux temperature extraction number extraction time particle size.The optimal extraction conditions were obtained as follows： three extraction cycles at 100 ℃ for 2 h each with a solid/liquid ratio of 1：30（g/mL）.Under these conditions,the yield of polysaccharides from Trichoderma pseudokoningii was（11.09 ± 0.13）%.Conclusion： The optimized process is stable,feasible and applicable for the extraction of polysaccharides from Trichoderma pseudokoningii mycelium.