超强磁性微球提取深加工转基因食品DNA

A DNA Isolation Method for Deeply Processed Transgenic Foods Based on High-Magnetic Response Microspheres

将磁性微球提取基因组核酸方法与实时荧光定量聚合酶链式反应(qRT-PCR)相结合,建立食品中转基因成分快速检测的新方法。以大豆和马铃薯的深加工食品为研究对象,考察磁性微球对基因组核酸的提取效率。通过聚合酶链式反应(PCR)和qRT-PCR对所提取的DNA样品进行分析,并与市售试剂盒等方法进行比较。结果表明,本方法提取的DNA模板的完整性、纯度、可扩增性以及qRT-PCR反应适用性均良好,甚至超过市售试剂盒的提取效果,特别适用于大豆油和马铃薯淀粉等深加工食品中的基因片段提取。

DNA extraction is the difficulty in detecting genetically modified（GM） foods.DNA extraction with highmagnetic response microspheres coupled with qRT-PCR has been applied to establish a rapid new method for the detection of transgenic components in foods.The extraction efficiency of geomic DNA from deeply processed products of potato and soybean with high-magnetic response microspheres was investigated in this study.The DNA samples obtained were analyzed by PCR and qRT-PCR and the results were compared with those obtained with a commerical kit.Complete,high pure,amplifiable DNA templates which were suitable for qRT-PCR were obtained by the proposed method and even its extraction efficiency was better than that of the commercial kit.Therefore,this method is particularly applicable for extracting DNA fragments from deeply processed products of potato and soybean.