南海不同产地近江牡蛎中牛磺酸含量检测

Determination of Taurine in Ostrea rivularis from Different Parts of the South China Sea

建立牡蛎中牛磺酸含量检测的高效液相色谱方法。近江牡蛎样品经超声波处理,所得样液采用丹磺酰氯柱前衍生后C18色谱柱分离,紫外检测器定量检测,方法检出限1.2×10-5μg/mL,牛磺酸质量浓度在0.2～2.2μg/L范围内线性关系良好,R2为0.9932,方法加标回收率92.24%～96.73%。样液采用Amino-Na色谱柱分离,邻苯二甲醛(OPA)柱后衍生后荧光检测器检测,方法检出限0.022μg/mL,牛磺酸质量浓度在0.2～2.2μg/mL范围内线性关系良好,R2为0.9905,方法加标回收率为92.14%～96.73%。两种方法均具有较高的重现性和稳定性,OPA柱后衍生法精密度(RSD为0.81%)高于丹磺酰氯法(RSD为6.42%)。采用这两种方法分别检测南海不同产地近江牡蛎中的牛磺酸含量,结果无显著性差异,说明两种方法均适用于牡蛎等贝类样品中牛磺酸的检测。

An HPLC method for the determination of taurine in Ostrea rivularis was established.The optimal conditions of experimental parameters such as reaction temperature and mobile phase flow rate were established.The HPLC method was validated with respect to accuracy,precision and limit of quantification.When Ostrea rivularis samples were extracted by a ultrasonic-assisted extraction method,the resulting extract was separated on a C18column following pre-column derivatization with dansyl chloride,and quantitative detection was performed with a UV detector,the developed standard curve of taurine was linear over the range of 0.2 to 2.2 μg/L,with correlation coefficient（R2） of 0.9932,the limit of detection（RSN= 3） was 1.2 × 10-5μg/mL,and the spike recoveries were between 92.24% and 96.73%,with relative standard deviation（RSD） of 6.42%.When the HPLC method was based on separation on an Amino-Na column followed by post-column derivatization with orthophthalaldehyde and fluorescence detection,the proposed standard curve of taurine was linear over range between 0.2 μg/mL and 2.2 μg/mL（R2 = 0.9905）,the limit of detection（RSN = 3） was 0.022 μg/mL,and the spike recoveries were between 92.14% and 96.73%,with RSD of 0.81%.In conclusion,the two methods are simple,rapid,reliable and high accurate and precise and can be used to determine taurine in Ostrea rivularis from different parts of the South China Sea with no significant differnce.