高效液相色谱法测定白葡萄汁中苯甲酰脲类农药

Determination of Benzoylurea Insecticides in White Grape Juice by High Performance Liquid Chromatography

以二氯甲烷为萃取剂,甲醇为分散剂,采用分散液液微萃取-正相高效液相色谱法测定白葡萄汁中4种苯甲酰脲类农药(氟苯脲、杀铃脲、除虫脲、氟铃脲)。色谱条件:Apollo Silica柱;正己烷-无水乙醇(97:3,V/V)为流动相;流速:1.0mL/min;紫外检测波长:200nm。在0.33～100μg/mL范围内4组分线性良好,方法检出限在5.80～6.60ng/mL之间,平均回收率范围是73.72%～97.85%,相对标准偏差范围是1.33%～4.62%。该方法快速、检出限低、重现性好,可用于白葡萄汁中苯甲酰脲类农药的测定。

Residues of benzoylurea insecticides（teflubenzuron,triflumuron,diflubenzuron and hexaflumuron） in white grape juice were determined by dispersive liquid-liquid micro-extraction combined with normal-phase high performance liquid chromatography.In the dispersive liquid-liquid micro-extraction,the extraction solvent was methylene chloride and dispersive solvent was methanol.The analytes were separated on Apollo Silica column with a mobile phase made up of n-hexane and anhydrous alcohol（97：3,V/V） at a flow rate of 1.0 mL/min and detected at 200 nm.Good linearity was found in the range of 0.33 to 100 μg/mL with correlation coefficients above 0.999.The limits of detection were between 5.80 ng/mL and 6.60 ng/mL.The average recoveries of the benzoylurea insecticides ranged from 73.72% to 97.85%,with relative standard deviations between 1.33% and 4.62%.The proposed method is rapid and highly reproducible and shows a limit of detection.Therefore it is applicable for the determination of benzoylurea insecticide residues in white grape juice.