PCR-ELISA法检测食品中空肠弯曲菌

Rapid Detection of Campylobacter jejuni in Foods by PCR-ELISA

针对空肠弯曲菌16S rRNA基因应用Primer Express 3.0软件设计特异性引物和探针,核酸探针5’端标记生物素,将聚合酶链式反应(PCR)、核酸液杂交以及酶联显色技术结合,对检测条件进行优化,建立食品中空肠弯曲菌的聚合酶链式反应-酶联免疫吸附分析法(PCR-ELISA)。该方法特异性强、灵敏度高(比PCR高10倍),可以快速、准确检测食品中污染的空肠弯曲菌。该方法可应用于临床诊断、食品卫生监控及出口食品的病原微生物检测等各个领域。

A PCR-ELISA method was developed to detect Campylobacter jejuni contamination in foods through the combined use of PCR,nucleic acid hybrid reaction and enzyme coloration.A forward PCR primer and a probe labeled with biotin at the 5’ end was designed from Campylobacter jejuni 16S rRNA gene sequence by using Primer Express Software 3.0.The testing conditions were optimized and developed for PCR-ELISA detection of Campylobacter jejuni in foods.The PCRELISA method revealed 10 times higher sensitivity than the ordinary PCR method.PCR-ELISA has potential applications in such fields as clinic diagnosis,food hygiene monitoring and pathogen detection in food imports and exports.