真空包装罗非鱼冷藏过程生物胺生成与微生物生长的相互关系

Relationship of Biogenic Amines with Microbial Growth of Vacuum-Packed Tilapia during Cold Storage

通过测定真空包装罗非鱼冷藏过程生物胺和主要微生物的变化,建立两者的相关性和回归方程,以探讨生物胺生成与微生物生长的相互关系。结果表明:冷藏过程中,腐胺、尸胺、组胺是优势生物胺;色胺、苯乙胺、酪胺、亚精胺、精胺与微生物生长不相关,其他生物胺指标与微生物生长具有良好相关性;二胺、生物胺指数(BAI)能有效反映总生物胺的变化趋势,用于品质评价更为简便。通过逐步回归分析,获得贮藏时间与生物胺、微生物具有良好拟合度的多元线性回归方程:贮藏时间=0.125×组胺+0.941×厌氧菌+0.022×尸胺-2.918。

Changes in biogenic amines and major microflora in vacuum-packed tilapia during cold storage were investigated to reveal the relationship between biogenic amines formation and microbial growth by correlation analysis with regression equation.The results showed that,during cold storage,the dominant biogenic amines were putrescine,cadaverine and histamine while no signficant correlations were observed between microbial growth and biogenic amines such as tryptamine,phenylethylamine,tyramine,spermidine and spermine while the other biogenic amine indicators showed good correlation with microbial growth.The diamine and biogenic amines index（BAI） could be used to reflect the change trend of total biogenic amines effectively which were simple for quality evaluation.Using stepwise regression analysis,a well-fitted multivariate linear regression equation indicating storage days with respect to biogenic amines and microbial growth was obtained as follows： storage days = 0.125 histamine＋0.941 anaerobe＋0.022 cadaverine-2.918.