摘要
目的:研究真空气流细胞破壁技术(VAPB)对桑叶中生物碱、黄酮及多糖提取率的影响。方法:采用HPLC法,分别以1-脱氧野尻霉素及芦丁为对照品,测定破壁与未破壁桑叶中的生物碱及黄酮含量;采用分光光度法,以葡萄糖为对照品,测定了破壁与未破壁桑叶中的多糖的含量,并比较测定值。结果:破壁较未破壁桑叶样品中的生物碱、黄酮及多糖含量的测定值分别高出74%、58%和48%。结论:采用VAPB对桑叶进行提取前处理,可提高其有效成分的溶出率,更好地发挥其生物活性。
Objective： To investigate the effect of VAPB on the extraction efficacy of alkaloids,flavonoids and polysaccharides in mulberry leaves.Methods： The contents of alkaloids,flavonoids and polysaccharides in mulberry leaves with damaged cell wall and intact wall were determined and compared by HPLC or spectrophotometric methods.Results： The contents of alkaloids,flavonoids and polysaccharides in mulberry leaf samples with damaged cell wall were higher than those with intact cell wall,which were 74%,58% and 48% respectively.Conclusions： Pretreatment of mulberry leaves by VAPB may significantly increase the solubility of the active components in mulberry leaves.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第10期327-330,共4页
Food Science
基金
吉林省科技厅科技发展计划项目(20100938)
关键词
桑叶
真空气流细胞破壁
生物碱
黄酮
多糖
提取率
mulberry leaves
vacuum air current for plant cell wall breakdown
alkaloid
flavonoid
polysaccharide
extraction
