盐酸小檗碱对肉兔脂质代谢、内分泌及抗氧化功能的影响

Effect of Berberine Hydrochloride on Lipid Metabolism,Endocrine and Antioxidan Function of Meat Rabbit

本试验旨在研究盐酸小檗碱对肉兔脂质代谢、内分泌及抗氧化功能的影响。采用单因子随机区组试验设计,选择128只50日龄且体重相近的健康新西兰兔,随机分为4组,1个对照组和3个处理组,每组32个重复,每个重复1只兔。对照组饲喂基础饲粮,3个处理组分别在基础饲粮上添加10、20及30mg.kg-1的盐酸小檗碱。试验期为34d,其中预饲期为7d,正试期为27d。结果表明:随着小檗碱添加量的提高,背最长肌和肝脏粗脂肪、甘油三酯和总胆固醇含量及血清甘油三酯、总胆固醇和低密度脂蛋白胆固醇浓度逐渐降低,而血清高密度脂蛋白胆固醇浓度及肝脏脂蛋白脂酶和肝脂酶活性逐渐升高;小檗碱不同添加量均可显著(P<0.05)或极显著(P<0.01)提高血清生长激素、三碘甲腺原氨酸和胰岛素水平及降低血清胰高血糖素水平和葡萄糖浓度,而20和30mg.kg-1添加量可极显著(P<0.01)提高血清四碘甲腺原氨酸水平;随着小檗碱添加量的提高,背最长肌、肝脏和血清总超氧化物歧化酶活性逐渐升高,而丙二醛浓度逐渐下降。研究结果提示:饲粮添加小檗碱可改善肉兔脂质代谢,调控内分泌系统,提高机体抗氧化功能。

The aim of the study was to investigate the effects of berberine hydrochloride on lipid metabolism, endocrine and antioxidan function of meat rabbit. 128 New Zealand rabbits with 50-day-old and similar body weight were randomly allotted into four groups. One group was the control group and the others were the treating groups. There were thirty-two replicates in each group and one rabbits were used in per replicate. The experiment was designed by single factor random arrangement. The rabbits in the control group were fed with the basal diet. And the rabbits in the three treating groups were fed the basal diets with 10, 20 and 30 mg·kg-1 berberine hydrochloride supplementation, respectively. The whole experiment lasted thirty-four days. The duration of the preliminary experiment was 7 d and the duration of the formal experiment was 27 d. The results showed that： with increased supplementation level of berberine, the concentration of ether extract, triglyceride and total cholesterol in longissimus dorsi and liver and the concentration of triglyceride, total cholesterol and low-density lipoprotein cholesterol in serum decreased, and the concentration of high-density lipoprotein cholesterol in serum and the activity of lipoprotein lipase and hepatic lipase in liver increased; every supplementation level of berberine increased the level of growth hormone, triiodothyronine and insulin in serum and decreased the level of glucagon and the concentration of glucose in serum （P〈0.05 or P〈0.01）; the 20 and 30 mg·kg-1 supplementation levels of berberine increased the level of tetraiodothyronine in serum（P〈0.01）; with increased supplementation level of berberine, the activity of total superoxide dismutase increased and the concentration of maleic dialdehyde decreased. The results suggest that： with the supplementation of berberine to the diet of meat rabbits, the lipid metabolism could be improved, the endocrine system could be regulated and controlled, and the antioxidan function could be enhanced.