宫颈鳞癌RUNX3基因启动子区甲基化及其临床意义

Promoter methylation of RUNX3 gene in cervical squamous cell carcinoma and its clinical significance

目的研究宫颈鳞状细胞癌(鳞癌)组织中RUNX3基因启动子甲基化状态及临床意义。方法采用甲基化特异性聚合酶链式反应(MSP)测定50例宫颈鳞癌(CSCC组)、50例宫颈上皮内瘤变(CIN组)手术标本及10例正常宫颈组织(对照组)中RUNX3基因的甲基化状态,分析RUNX3基因甲基化与宫颈鳞癌临床病理学特征的关系。结果 CSCC组和CIN组中分别有27例(54%)和17例(34%)存在RUNX3基因启动子甲基化,而对照组未检出;三组间RUNX3基因启动子甲基化的发生率比较差异有统计学意义(χ2=11.500,P<0.005),CIN不同级别组(CINⅠ、Ⅱ、Ⅲ级)的RUNX3基因启动子甲基化发生率比较差异有统计学意义(χ2=9.655,P<0.05)。RUNX3基因启动子甲基化与宫颈鳞癌的分化程度及是否发生淋巴结转移有关(r=-0.367,P=0.017;r=0.288,P=0.042)。结论 RUNX3作为抑癌基因参与宫颈鳞癌的发生和发展,可作为对宫颈鳞癌患者进行预后评估的生物学指标。

Objective To determine the status of promoter methylation of RUNX3 gene in cervical squamous cell carcinoma and its clinical significance. Methods Methylation-specifie PCR （MSP） was employed to detect the status of promoter methylation of RUNX3 gene in 50 samples of cervical squamous cell carcinoma （CSCC group）, 50 samples of cervical intraepithelial neoplasia （CIN group）, and 10 samples of normal cervical tissues （control group）. The relationship between promoter methylation of RUNX3 gene and clinicopathological characteristics of cervical squamous cell carcinoma was explored. Results Promoter methylation of RUNX3 gene was found in 27 cases （54%） in CSCC group and 17 cases （34%） in CIN group, while none was observed in control group. There were significant differences in the incidences of promoter methylation of RUNX3 gene among three groups （X2 = 11. 500, P 〈 0. 005）, and there were significant differences in the incidences of promoter methylation of RUNX3 gene among CIN I group, CIN ]I group and CIN 1I[ group （ X2 = 9. 655, P 〈 0.05）. The promoter methylation of RUNX3 had a significant correlation with differentiation of cervical squamous cell carcinoma and lymph node metastasis （ r = - 0. 367, P = 0. 017; r = 0. 288, P = 0. 042）. Conclusion RUNX3 may function as a tumor suppressor gene in the oncogenesis of CSCC and may serve as a biological indicator of prognosis.