不同基质和pH值的腐植酸钠凝胶剂对大鼠皮肤创伤愈合的影响

Effects of Sodium Humic Gel with Different Matrix and pH on the Wound Healing of Rats

目的:研究不同基质和pH值的腐植酸钠凝胶剂对大鼠皮肤创伤愈合的影响。方法:分别用卡波姆、羧甲基纤维素钠(CMC-Na)、海藻酸钠3种基质制备腐植酸钠凝胶剂,并用三乙醇胺调节为低、高pH值。取大鼠建立背部创伤模型后,随机分为卡波姆组、CMC-Na组和海藻酸钠组,每组6只,分别在各组大鼠背部的左上、左下、右上、右下侧创面涂抹生理盐水(空白对照组)、空白基质凝胶(基质对照组)、相应基质的低pH值腐植酸钠凝胶剂和高pH值腐植酸钠凝胶剂,每次每个创面0.5g,每日2次,连续给药14d;观察各组大鼠分别给药0、3、6、9、14d后的创面愈合情况,并计算愈合率和平均愈合时间。结果:与空白对照组和基质对照组比较,卡波姆组、CMC-Na组和海藻酸钠组大鼠给药不同时间的愈合率均明显增加(P<0.01),平均愈合时间均明显缩短(P<0.01)。低pH组间比较或高pH组间比较,卡波姆组大鼠给药3、6、9、14d的愈合率均明显高于CMC-Na组和海藻酸钠组(P<0.05),平均愈合时间明显短于CMC-Na组和海藻酸钠组(P<0.05);基质相同时,高pH值凝胶剂对大鼠给药3、6、9、14d的愈合率明显高于低pH值凝胶剂(P<0.05),平均愈合时间明显短于低pH值凝胶剂(P<0.05)。结论:以卡波姆为基质制备的高pH值的腐植酸钠凝胶剂促进大鼠创伤愈合的效果更好。

OBJECTIVE：To study the effects of Sodium humic gel with different matrix and pH on the wound healing of rats.METHODS：Sodium humic gel was prepared with 3 kinds of matrix [carbomer,CMC-Na,sodium alginate],and pH value was adjusted with triethanolamine.After modeling,all rats were randomly divided into carbomer group,CMC-Na group and sodium alginate group with 6 rats in each group.On the upper left,bottom left,upper right and bottom right wound of the back of rats in each group,we daubed normal saline（blank control group）,blank gel matrix（matrix control group）,the high pH value of Humic acid sodium gel and the low pH value of Humic acid sodium gel,respectively.Each rat of all groups were daubed corresponding gels of 0.5 g twice a day for 14 d.The wound healing of rats were observed by 0,3,6,9 and 14 d after administration,and healing rate and mean healing time were calculated.RESULTS：Compared with blank control group and matrix control group,the healing rate of carbomer group,CMC-Na group and sodium alginate group increased significantly（P0.01）,while mean healing time shortened significantly（P0.01）.Compared between low pH value and high pH value,healing rate of carbomer group was higher than CMC-Na group and sodium alginate group 3,6,9 and 14 d after administration（P0.05）;mean healing time of carbomer group was much shorter than other two groups（P0.05）.In the same matrix gels,healing rate of gel with high pH value was significantly higher than that of gel with low pH value 3,6,9 and 14 d after administration（P0.05）;while mean healing time of gel with high pH value was much shorter than low pH value（P0.05）.CONCLUSIONS：Using carbomer as matrix,Humic acid sodium gel with high pH value has a better effect on the wound healing of rats.