HPLC法同时测定曲克芦丁香豆素片中曲克芦丁和香豆素的含量

Simultaneous Determination of Troxerutin and Coumarin in Troxerutin Coumarin Tablets by HPLC

目的:建立同时测定曲克芦丁香豆素片中曲克芦丁和香豆素含量的方法。方法:采用高效液相色谱法。色谱柱为Venusil C18,以0.1mol/L磷酸二氢钠溶液(用磷酸调节pH值至4.4)为流动相A,乙腈为流动相B,梯度洗脱,流速为0.5ml/min,检测波长为276nm。结果:曲克芦丁和香豆素检测质量浓度线性范围分别为1.00～200.98、1.05～42.19μg/m(lr均为0.9999),平均回收率分别为99.8%、99.5%,RSD分别为0.6%、0.8%(n=9)。结论:该方法简便、准确,可用于曲克芦丁香豆素片的质量控制。

OBJECTIVE：To establish a method for simultaneous determination of troxerutin and coumarin in Troxerutin coumarin tablets.METHODS：HPLC method was adopted.The determination was performed on Venusil C18 column with mobile phase consisted of 0.1 mol/L NaH2PO4 solution（pH adjusted to 4.4 with phosphoric acid）as mobile phase A and acetonitrile as mobile phase B（gradient elution）at the flow rate of 0.5 ml/min.The detection wavelength was 276 nm.RESULTS：The linear ranges were 1.00-200.98 μg/ml for troxerutin（r=0.999 9）and 1.05-42.19 μg/ml for coumarin（r=0.999 9）.The average recoveries were 99.8%（RSD=0.6%,n=9）and 99.5%（RSD=0.8%,n=9）.CONCLUSIONS：The method is simple and accurate,and suitable for quality control of Troxerutin coumarin tablets.