人脑胶质瘤组织MGMT和EGFR及Ki-67表达临床意义分析

Expression and clinical significance of MGMT,EGFR and Ki-67 in human gliomas

目的:探讨人脑胶质瘤组织中O6-甲基鸟嘌呤DNA甲基转移酶(MGMT)、表皮生长因子受体(EGFR)和细胞增殖相关核蛋白Ki-67的表达及其相关性。方法:选取64例脑胶质瘤组织标本,采用免疫组织化学染色法检测MGMT、EGFR及Ki-67的表达。结果:MGMT、EGFR、Ki-67总体阳性率分别为76.6%、59.4%和56.3%。在Ⅰ、Ⅱ、Ⅲ、Ⅳ级胶质瘤中,MGMT阳性表达率分别为0、58.8%、70.0%和100.0%;EGFR阳性表达率分别0、41.2%、60.0%和76.0%;Ki-67阳性表达率分别为0、23.5%、65.0%和76.0%。MGMT、EGFR和Ki-67的阳性表达率在脑胶质瘤不同病理分级间差异有统计学意义,P值分别为0.001、0.043和0.002;且三者的表达强度与胶质瘤病理分级呈正相关性,r值分别为0.315、0.273和0.400,P值分别为0.011、0.029和0.001。3种检测指标的阳性表达率与发病年龄及性别均无明显的相关性,差异无统计学意义,P>0.05。分层分析发现,Ki-67的表达与MGMT和EGFR的表达有相关性,r分别为0.296和0.466,P值分别为0.018和0.000。结论:人脑胶质瘤中MGMT、EGFR及Ki-67的表达与病理分级均有相关性,测定三者的表达水平可评价胶质瘤细胞的恶性程度,对胶质瘤预后判断可能有指导作用。

OBJECTIVE：To investigate the expression of O6-methylguanine-DNA-Methyltransferase （MGMT）,epi- dermal growth factor reeeptor（EGFR） and Ki-67 in human glioma cell, as well as the relations among the three in glioma. METHODS：Total of 64 samples of glioma were used to detect the expression of MGMT,EGFR and Ki 67 by using immu- nohistochemistry. RESULTS： The positive expression rate of MGMT,EGFR and Ki-67 in the glioma tissue were 76.6%, 59.4% and 56.3 %. In different grades（WHO Grade Ⅰ , Ⅱ , Ⅲ , Ⅳ） of glioma sample,the positive expression rate of MG- MT were 0,58.8% ,70.0% and 100.0% ; the positive expression rate of EGFR were 0,41.2% ,60.0% and 76.0% ; the positive expression rate of Ki-67 were 0,23.5% ,65.0% and 76.0%. The expression of MGMT,EGFR and Ki-67 were significantly different in pairwise comparison of every two grades of results among all of them,and P values were 0. 001, 0. 043 and 0. 002. They were correlated well with Pathological grades （MGMT r=0. 315,P--0. 011; EGFR r=0. 273, P= 0. 029; Ki-67 r= 0. 400, P= 0. 001）. There was no significant difference in the MGMT, EGFR and Ki-67 positive ex- pression of different age groups （P〉0.05）; The positive rate of MGMT,EGFR and Ki-67 expression between male and female was not significantly different （P〈0.05）. Further stratified analysis found that the expression of Ki-67 correlated well with each other （Ki-67 vs MGMT r=0. 296,P=0. 018; Ki-67 vs EGFR r=0. 466,P=0. 000）. CONCLUSIONS：The expression level of MGMT, EGFR and Ki-67 are bound up with the different Pathological grading, and multy-parameterdetection can become the standard in estimating histological grade and improve the veracity of forecasting the prognosis.