甲状腺素诱导小鼠模型表观指征变化与阴虚火旺证的相关性研究

Correlative Study Between the Exterior Signs of Mice Model Induced by Thyroxine and Hyperactivity of Fire due to Yin Deficiency Syndrome

目的考察甲状腺素诱导小鼠模型表观指征的变化与阴虚火旺证的相关性,为阴虚火旺证动物模型的构建提供参考。方法将48只体质量18～22 g小鼠随机分为空白对照组和3个模型组,每组12只,雌雄各半,模型组每天分别灌胃给药不同剂量(40mg/kg、80mg/kg、160mg/kg)的甲状腺片混悬液,空白对照组灌胃给药双蒸水,并测定小鼠体质量、摄食量、饮水量、体温、自发活动和心率的动态变化。结果与空白对照组相比较,各模型组小鼠均表现为体质量增加缓慢,摄食量与饮水量增加,体温升高,心率加快,自主活动频繁等特征,能够模拟形体消瘦,消谷善饥,口干欲饮,五心烦热,心悸,烦躁等阴虚火旺证的主要临床指征,其中高剂量模型组雌性小鼠表观指征的变化更显著,与空白对照组比较有显著性差异(P<0.01)。结论甲状腺素造模诱导小鼠表观指征的变化能够模拟阴虚火旺证的主要临床特征,雌性小鼠每天灌胃给药160mg/kg,连续14d诱导模型小鼠表观指征的变化与阴虚火旺证的相关性更为显著。

Objective To observe the correlation between the exterior signs of mice model induced by thyroxine and hyperactivity of fire due to yin deficiency syndrome to provide reference for constructing hyperactivity of fire due to yin deficiency syndrome animal model. Method 48 standard weight mice were randomly divided into control group and three model groups, 12 each group, male and female in half. Model groups were managed with thyroid tablet suspension of different doses （40 mg/kg, 80 mg/kg and 160 mg/kg） respectively, whereas the control group with double-distilled water. The body weight, food intake, water intake, body temperature, spontaneous activity and heart rate were measured dynamically. Result As compared with the control group, mice in the model groups manifested slowly increased body weight, enhanced food intake and water intake, elevated body temperature,quicken heart rate and frequent spontaneous activity, which could simulate the major clinical characteristics of hyperactivity of fire due to yin deficiency syndrome, such as emaciation, voracious appetite, thirsty for water, internal heat, palpitation, impatience, et al. Among which, the exterior signs of female mice in high-dose model group were extremely notable compared with the control group （P 〈 0.01 ）. Conclusion The exterior signs induced by thyroxine can simulate the major clinical characteristics of hyperactivity of fire due to yin deficiency syndrome, the correlation between the exterior signs of female mice model induced by thyroxine i.g. 160 mg/kg/d for consecutive 14 days and hyperactivity of fire due to yin deficiency syndrome is more significantly.