H22实体瘤小鼠生存实验及环磷酰胺对生存期的影响

Survival Test and Effect of Cyclophosphamide in H22 Solid Tumor-bearing Mice

目的研究腹水型H22肝癌皮下移植小鼠的生存期及环磷酰胺的影响。方法将H22肝癌腹水瘤细胞接种于BALB/c小鼠左侧腋窝皮下,5d后按肿瘤体积随机分成环磷酰胺组和对照组。环磷酰胺组腹腔注射环磷酰胺50mg/kg,每周2次。观察小鼠一般临床表现、成瘤时间、成瘤率、体质量、摄食量、饮水量、肿瘤体积及生存时间。结果 H22皮下移植小鼠成瘤时间约为5d,成瘤率100%。环磷酰胺组体质量、肿瘤体积与对照组比较明显降低(P<0.05),环磷酰胺组摄食量、饮水量与对照组比较有增加的趋势。H22皮下移植瘤小鼠平均生存时间为87d,环磷酰胺组小鼠平均生存时间为113d(生命延长率30.3%,P<0.01)。结论 H22腹水型肝癌皮下移植小鼠是一种较理想的实体瘤模型,具有正常的免疫功能,可用于生物反应调节剂抗肿瘤药物影响荷瘤小鼠的生存期实验。

Objective To study Survival time and Effect of Cyclophosphamide in H22 solid Tumor-bearing mice. Method H22 cells were implanted into the left axilla of 50 BALB/c mice. Turnout growth was observed. 5 days later, The mice were randomly divided into Cyelophosphamide group and the control group by tumor volume. Cyclophosphamide group intraperitoneal injection of cyclophosphamide 50mg/kg, 2 times a week. Observe the general clinical manifestations, tumor formation time, the rate of tumor formation, weight, food intake, water intake, tumor volume and survival time. Result The H22 tumour tubercles could be formed on day 5. The tumour-take rate was 100% （50/50）. The average survival time of H22 subcutaneous xenograft mice is 87 days. The average survival time of Cyclophosphamide group is l l3days （Life extension rate is 30. 3%, P 〈0. 01 ）. Conclusion H22 aseites hepatoma transplanted subcutaneously in mice is an ideal model of solid tumors, which have normal immune function, and can be used for the survival test of biological response modifier anticancer drugs.