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猪圆环病毒2型ORF2优化基因在酵母中的表达 被引量：1

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摘要 为了研究猪圆环病毒2型(PCV-2)Cap蛋白亚单位疫苗,试验采用优化过的ORF2基因,成功构建了酵母重组表达质粒pGAPZaA-ORF2,用BlnⅠ对其进行线性化,通过电击转化,将其整合入毕赤酵母X33基因组中,ZeocinTM抗性筛选获得转化子pGAPZaA-ORF2-X33,通过PCR、SDS-PAGE和Western-blot进行分析鉴定。结果表明:Cap蛋白在酵母菌中成功分泌表达。
作者 姜后全 郑红玉 项林盛 唐丽云 黄毓茂
机构地区 华南农业大学兽医学院
出处 《黑龙江畜牧兽医》 CAS 北大核心 2013年第6期109-111,共3页 Heilongjiang Animal Science And veterinary Medicine
基金 广东省现代生猪产业技术体系项目(5500-F10021)
关键词 猪圆环病毒2型 ORF2基因 毕赤酵母 分泌表达
分类号 S852.659.2 [农业科学—基础兽医学]
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参考文献5

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共引文献8

同被引文献33

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黑龙江畜牧兽医
2013年 第6期

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