摘要
为了制备高质量的兔抗梅花鹿生茸区骨膜细胞裂解液血清,用于免疫学筛选cDNA表达文库,试验采用淋巴结注射和背部皮下注射的方法制备了兔抗梅花鹿生茸区骨膜细胞裂解液血清。结果表明:制备的抗血清效价是1∶128,质量较高。
To prepare high -quality rabbit serum against the lysates of antlerogenic periusteal cells from sika deer for immunological screening cDNA expression library, the methods of lymph node injection and back subcutaneous injection were used for preparing the antiserum. The result showed that the antiserum titer was 1：128, showing high quality.
出处
《黑龙江畜牧兽医》
CAS
北大核心
2013年第6期153-155,共3页
Heilongjiang Animal Science And veterinary Medicine
基金
"973"前期研究专项项目(2011CB111515)
国家自然科学基金项目(31070878)
吉林省自然科学基金项目(20101575)
关键词
血清
生茸区骨膜细胞
裂解液
serum
antlerogenic pefiosteal cells
lysate
