固体过氧化氢的制备及杀菌效果研究

Preparation of solid hydrogen peroxide and research of germicidal test

以无水硫酸钠、30%过氧化氢(H2O2)溶液和氯化钠为原料制备固体H2O2;采用单因素试验设计法考察了原料配比、反应温度、反应时间及干燥温度对产品产量及产品中H2O2含量的影响,并据此确定最佳制备条件;采用微生物悬液定量杀菌试验考察了所制备固体H2O2的抗菌活性。结果显示:在25 mL 30%H2O2溶液中,加入32 g硫酸钠、4 g氯化钠,反应时间30 min,反应温度40℃,干燥温度60℃时得到的产物H2O2含量最高,产品质量最好。10.8 g.L-1的H2O2消毒液对大肠杆菌、金黄色葡萄球菌作用10 min的平均杀菌率达到99.9%,对蜡样芽孢杆菌作用1 h的平均杀菌率达到100%。结论:本试验制备的固体H2O2具有良好的杀菌效果,且制备工艺简单。

Solid H2 O2 was prepared by anhydrous sodium sulfate ,30% H2O2 solution and sodium chloride. Using the single-factor ex- perimental design method, the impact of the material ratio, reaction temperature, reaction time, drying temperature on product yield and H2O2 content was studied to determine the optimum preparation technology. In addition,suspension quantitative germicidal test was carried out to know the germicidal efficacy. The results showed that the product was of a higher H2 O2 content and good quality in the following condition,25 mL 30% H2 O2,adding 32 g NaSO4 ,4 g NaCl,with the reaction time of 30 minutes,reaction temperature of 40 ℃, and the drying temperature of 60 ℃. 10. 8 g . L-1 H2O2 thimerosal killed 99.9% in average of Escherichia coli, Staphylococcus aureus with a 10 rain contact and 100% in average of Bacillus cereus with a 1 h contact. Conclusion： solid H2O2prepared in this test has a good bactericidal effect,and the preparation process is simple.