鸡肉中阿德呋啉的残留检测——高效液相色谱法

Determination of adprin content in chichen by HPLC

建立了检测鸡肉中阿德呋啉药物残留的高效液相色谱法(HPLC)。样品经氯仿提取,MCX固相萃取柱净化,XBD-C18色谱柱(4.6 mm×250 mm,5μm)分离,以甲醇水溶液(体积分数50%)为流动相,260 nm处检测。方法在100～500 ng.mL-1范围内线性关系良好(R2≥0.999),检出限为25μg.kg-1。阿德呋啉在80、100、150和200μg.kg-14个添加水平时,平均回收率分别为61.4%、70.4%、88.4%和90.2%,相对标准偏差(RSD)小于10%。结论:该方法快速、灵敏、高效,适用于鸡肉中阿德呋啉残留的测定。

A method for the determination of adprin in chicken by high performance liquid chromatography（HPLC） was developed. The adprin in the samples were first extracted with chloroform, and then the extraction was purified and concentrated by MCX cartridges. The analyses were performed on HPLC with an C18 column（4.6 min×250 mm,5 μm）,and the mobile phase was methanol-ware. This method showed a good linearity. For adprin,the linearity was in the range of 100-500 ng.mL-1. The detection limits of adprin were 25 μg.kg-1. At the spiked levels of 80,100,150 and 200μg. kg-1, the average recoveries of adprin were 61.4% 70.4% ,88.4% and 90. 2% ,respectively. The relative standard deviations were less than 10%. Conclusion： the proposed separation and determination method is rapid, sensitive and efficient, and that it is practical for the determination of adprin in chicken.