抗柯萨奇B病毒性心肌炎胶囊复方新制剂体外抗柯萨奇B_3病毒作用

Antiviral Effect of New Compound Anti-Coxsackievirus B Myocarditis Capsule on Coxsackievirus B_3:in Vitro Study

目的研究抗柯萨奇B病毒性心肌炎胶囊复方新制剂对柯萨奇B3病毒(CVB3)感染的心肌细胞的保护作用及其作用机制。方法根据抗柯萨奇B病毒性心肌炎胶囊复方新制剂和利巴韦林最大无毒浓度(TC0)筛选4个实验浓度。将生长良好的H9C2细胞种在96孔板中,分为复方新制剂组、利巴韦林组、正常对照组和病毒对照组。通过4种不同的加药和加病毒方式,用MTT法观察不同浓度药物对细胞增殖的影响和对细胞保护、直接抗病毒、抗病毒吸附作用,结果用OD值表示。结果不同浓度复方新制剂组和利巴韦林组OD值均高于病毒对照组(P<0.05或P<0.01)。复方新制剂组中浓度为0.390625mg/ml的细胞增殖OD值高于浓度为0.1953125、0.09765625mg/ml及利巴韦林组各浓度(P<0.05或P<0.01);复方新制剂组中浓度为0.78125mg/ml的细胞保护OD值高于其他浓度,并且明显高于利巴韦林组各浓度(P<0.05或P<0.01)。复方新制剂组和利巴韦林组各浓度直接抗病毒和抗病毒吸附作用OD值比较差异均无统计学意义(P>0.05)。结论抗柯萨奇B病毒性心肌炎胶囊复方新制剂通过抑制病毒的增殖、保护细胞、直接抗病毒、抗病毒吸附等途径达到保护心肌细胞作用。其中浓度为0.390625mg/ml时抑制病毒增殖作用最明显,浓度为0.78125mg/ml时,对细胞保护作用最明显,并优于利巴韦林。

Objective To research the protective effect and mechanism of new compound Anti-Coxsackievirus B Myocarditis Capsule（ACMC）on cardiomyocytes infected by Coxsackievirus B3（CVB3）.Methods Four experimental concentrations were selected according to the maximum non-toxic concentrations（TC0）of ACMC and ribavirin.The well-grown H9C2cells were seeded in 96-well plates and divided into the ACMC group,ribavirin group,normal control group and virus control group.The effects of ACMC and ribavirin at different concentrations on cell proliferation,cell protection,direct anti-virus,anti-virus adsorption were observed by methyl thiazolyl tetrazolium（MTT）assay and indicated as OD values according to four-different dosing and virus-plus methods.Results The OD values at different concentrations in the ACMC and ribavirin groups were significantly higher than those in the model control group（P〈0.05or P〈0.01）.The OD value of cell proliferation at 0.390625mg/ml in the ACMC group was significantly higher than that at 0.1953125mg/ml and 0.09765625mg/ml in the ACMC group and at various concentrations in the ribavirin group respectively（P〈0.05or P〈0.01）.The OD value of cell protection at 0.78125mg/ml in the ACMC group was significantly higher than that at other concentrations in the ACMC group and at various concentrations in the ribavirin group respectively（P〈0.05or P〈0.01）.There was no significant difference between the ACMC group and ribavirin group in the OD values of direct anti-virus and anti-virus adsorption at various concentrations（P〈0.05）.Conclusion ACMC can protect the cardiomyocytes through inhibiting the virus proliferation,protecting the cells,directly fighting the virus and preventing the virus adsorption,with best inhibition of virus proliferation at 0.390625 mg/ml and protection of cells at 0.78125mg/ml and superior to ribavirin.