摘要
应用抗人肺癌单克隆抗体Lc-1对26例支气管粘膜活检标本进行ABC法免疫组织化学观察。活检标本采用2次包埋作连续冰冻切片,单抗Lc-1采用4个浓度100μg/ml,50μg/ml、25μg/ml、10μg/ml进行标记。结果表明Lc-1对不同病理类型的肺癌活检标本都有较强的结合能力,而对粘膜炎症无阳性反应,故可作为肺癌病理诊断与鉴别诊断主要辅助手段之一、在25～50μ/ml浓度标记效果很好,而在100μg/ml和10μg/ml浓度则有阴性结果出现。
An immunohislochemical study of McAb Lc-1 against human lung cancer was performed by ABC .method in 2G bronchoscopic biopsy specimens. Serial frozen sections were cut in the double embedding biopsy specimens by methyl cellulose ( OCT ). Four different concentrations of McAb Lc-1 were used in the immuno-enzyme labelling reactions; 100 μg/ml, 50 μg/ml, 25 μg/ml, and 10 μg/ml. The results demonstrated strong positive reactions of McAb Lc-1 to lung cancer specimens of various pathologic types, but no positive reactions to mucosal inflammatory lesions. Terefore, McAb Lc-1 can be used as an important assistant tool for making pathologic and differential diagnosis of lung cancers. The labelling results were rather good in the concentration ranging from 25 to 50 μng/ml, but negative results were observed in some of the 100 μg/ml and 10 μg/ml concentrations
基金
上海市科学发展基金
关键词
肺肿瘤
单克隆抗体
诊断
human lung cancer
McAb Lc-1
bronchoscopic biopsy specimen
