三氯乙烯作业人群尿中小分子蛋白水平研究

Study on urinary micro-protein among occupational trichloroethylene exposed workers

目的探讨三氯乙烯(TCE)职业接触对尿中3种小分子蛋白水平的影响。方法以112名TCE接触工人和70名对照作为调查对象,收集职业史、年龄、性别、吸烟和饮酒等信息。收集班后尿,测定三氯乙酸(TCA)水平及α1微球蛋白(α1-M)、β2微球蛋白(β2-M)、视黄醇结合蛋白(RBP)的水平。结果3种小分子蛋白α1-M、β2-M、RBP水平在两组调查对象中比较,差异均无统计学意义。以作业工龄、TCE浓度、尿中TCA水平分组后,作业工龄≥1.9a的工人α1-M水平明显高于对照组,差异有统计学意义(P<0.05);高TCE浓度(198.0～257.0mg/m3)组及尿中TCA水平≥50mg/L组的工人RBP水平均明显高于对照组,差异有统计学意义(P<0.05)。结论长时间和高水平TCE职业接触可致作业工人尿中α1-M、RBP水平增高。

Objective To investigate the effects of occupational trichloroethylene（TCE）exposure on the levels of 3 urinary micro-proteins.Methods 112 TCE exposed workers and 70 controls were recruited.Occupational history,age,gender,smoking and alcoholic habits of all subjects were collected by questionnaire.Trichloro-acetic acid（TCA）level and the levels of urinary α1-microglobulin（α1-M）,β2-microglobulin（β2-M）,and retinal binding protein（RBP）were measured.Results The distributions of age,gender,smoking and alcoholic habits were not significantly different between TCE exposed workers and controls.The differences in levels of urinary α1-microglobulin（α1-M）,β2-microglobulin（β2-M）,and RBP in both exposed and control groups were not statistically significant.When the subjects were grouped according to working age,exposure concentration and levels of urinary TCA,the level of α1-M in workers with working age≥1.9a was significantly higher than that of the controls;the difference was significant（P0.05）.In high TCE exposure concentration（198.0~257.0 mg/m3）and urinary TCA≥50 mg/L group,the RBP levels were significantly higher than those of the controls,the difference was significant（P0.05）.Conclusions Long-term and high level TCE exposure could induce the increase of urinary α1-M and RBP levels among occupational exposed workers.