奶牛布鲁氏菌病微量凝集试验诊断方法的建立

Establishment of Microscale Agglutination Test for Diagnosis of Cow Brucellosis

为探索操作简便、快速、实用的奶牛布病诊断方法,本研究建立了奶牛布鲁氏菌病(布病)诊断96孔V型板微量凝集方法,并优化了试验条件。结果显示,抗原的最佳稀释浓度为1∶20,反应最适温度为37℃,反应时间为24h;为验证本研究建立的微量凝集方法的准确性,对120份奶牛布病虎红平板凝集试验阳性血清样品进行微量法凝集和试管凝集试验,结果显示,微量法与虎红平板凝集试验的符合率为83.3%,试管法与虎红平板凝集试验符合率为80.0%。提示,微量凝集试验可以作为试管凝集试验的替代方法,应用于规模化奶牛场布病的诊断和检疫。

To explore simple,fast and practical diagnosis method for cow brucellosis in this study,we established a microscale agglutination test using 96-well ＂V＂ plate and optimized the test conditions,the optimal antigen diluted concentration was 1∶20,the optimal reaction temperature was 37 ℃,and the optimal reaction time was 24 h.To verify the veracity microscale agglutination test,we retested 120 rose-bengal plate agglutination test（RBT） positive cow serum samples by microscale agglutination test and tube agglutination test,the coincidence rate of microscale agglutination test and tube agglutination test to RBT were 83.3% and 80.0%,respectively.These results showed that the microscale agglutination test could be used as the alternative method of tube agglutination test for cow brucellosis diagnosis and quarantine.