超高效液相色谱测定猪配合饲料中苯乙醇胺A的研究

Determination of Phenylethanolamine A in Formula Feed by Using Ultra Performance Liquid Chromatography

研究建立了超高效液相色谱(ultra performance liquid chromatography,UPLC)—二极管阵列检测器(PDA)测定配合饲料中苯乙醇胺A的方法。通过应用0.1%甲酸甲醇溶液提取、混合阳离子固相萃取净化及样品基质校正外标定量方法进行测量。在优化条件下,苯乙醇胺A在0.1～20.0mg/kg浓度范围内线性关系良好,线性相关系数(R2)达到0.9945;在1.0、5.0、10.0mg/kg等不同添加浓度下,方法回收率高于90.36%,变异系数低于5.98%;方法检测限为0.06mg/kg,定量限为0.10mg/kg。结果表明,该方法灵敏度高、精密度好、准确可靠且操作简单,能够满足配合饲料中苯乙醇胺A的测定需要。

A rapid method for determination of phenylethanolamine A in formula feed by using ultra performance liquid chromatography（UPLC）-PDA had been developed.Phenylethanolamine A in formula feed was extracted with methanol containing 0.1% formic acid,followed mix cation ionic exchange solid phase extraction cartridge clean up,through the sample matrix correction external standard got quantitative.In the optimized conditions,a good linearity of phenylethanolamine A in feed sample matrix was obtained at the concentrations from 0.1 to 20.0 mg/kg,the linear relative coefficient of calibration curve（R2） was 0.9945.The recovery of phenylethanolamine A spiked in formula feed at different concentrations was above 90.36%,and the coefficient of variation was below 5.98%.The limit of detection was 0.06 mg/kg and the limit of quantification was 0.10 mg/kg.The method provided advantages of high sensitive,good precision,reliable data,easy operation and the potential use for rapid determination of phenylethanolamine A for supervising formula feed.