脂多糖诱导持续血小板减少模型的建立及对血小板生成素的影响

Establishment of A Continuing Thrombocytopenia Model Induced by Lipopolysaccharide

目的建立脂多糖(LPS)诱导小鼠持续内毒素血症血小板减少(TCP)模型,观察小鼠模型平均血小板体积(MPV)、血小板生成素(TPO)的变化。方法 C57/BL小鼠随机分为4组,低剂量LPS5mg/kg连续给药组(LL-c组),低剂量LPS5mg/kg单次给药组(LL组),高剂量LPS50mg/kg单次给药组(HL组),对照组(C组);于给药前测定各组小鼠血小板计数(PLT)和MPV作为基线,观察给药后小鼠PLT、TPO、MPV水平及存活情况。结果 LL-c组小鼠注射LPS后PLT下降,并持续处于基线30%左右的水平,与基线相比较差异均有统计学意义(P<0.01),LL组与HL组均先降低后升高;LL-c组注射LPS后MPV持续处于增大水平,与基线相比较差异有统计学意义(P<0.01),HL、LL组MPV均先增大后减小;7d时LL-c组小鼠TPO高于C组(P<0.05);LL-c组、LL组与HL组小鼠造模7d时分别死亡5只、0只、14只。结论连续低剂量腹腔注射LPS可诱导小鼠持续TCP模型。

Objective To establish a model of mouse continuing endotoxemia thrombocytopenia （TCP） induced by li- popolysaccharide （LPS）, observe changes of the mean platelet volume （MPV） and thrombopoietin （TPO） in this model. Meth- ods C57/BL mice were randomly divided into four groups, LPS low dose （5 mg/kg） of continue injection （LL-c） group, LPS low dose （5 mg/kg） of single injection （LL） group, LPS high dose （50 mg/kg） of single injection （HL） group and physiological sa- line injection （control） group. The blood platelet count （PLT） and MPV were detected before injection as the base line. The val- ues of PLT, TPO, MPV and mortality were observed after treatment. Results The value of PLT was significantly lower in LL-c group than that of basic line （P 〈 0.01）, which was continued in the baseline level of about 30%. The PLT levels were lower and then higher in HL and LL groups. The value of MPV was significantly higher in LL-c group than that of basic line （P 〈 0.01）, which was augmentation and then diminution in HL group and LL group. The TPO level of 7-day was significantly higher in LL-c group than that of control group （P 〈 0..05）. The mortality were respectively 5 rats, 0 rat and 14 rats in LL-c group, LL group and HL group. Conclusion The model of continuing endotoxemia TCP can be induced by consecutive low dose LPS intraperitoneal injection.